| RRC ID |
54597
|
| Author |
Fujimori T, Matsuda R, Suzuki M, Takenaka Y, Kajiura H, Takeda Y, Ishimizu T.
|
| Title |
Practical preparation of UDP-apiose and its applications for studying apiosyltransferase.
|
| Journal |
Carbohydr Res
|
| Abstract |
UDP-apiose, a donor substrate of apiosyltransferases, is labile because of its intramolecular self-cyclization ability, resulting in the formation of apiofuranosyl-1,2-cyclic phosphate. Therefore, stabilization of UDP-apiose is indispensable for its availability and identifying and characterizing the apiosyltransferases involved in the biosynthesis of apiosylated sugar chains and glycosides. Here, we established a method for stabilizing UDP-apiose using bulky cations as counter ions. Bulky cations such as triethylamine effectively suppressed the degradation of UDP-apiose in solution. The half-life of UDP-apiose was increased to 48.1 ± 2.4 h at pH 6.0 and 25 °C using triethylamine as a counter cation. UDP-apiose coordinated with a counter cation enabled long-term storage under freezing conditions. UDP-apiose was utilized as a donor substrate for apigenin 7-O-β-D-glucoside apiosyltransferase to produce the apiosylated glycoside apiin. This apiosyltransferase assay will be useful for identifying genes encoding apiosyltransferases.
|
| Volume |
477
|
| Pages |
20-25
|
| Published |
2019-5-15
|
| DOI |
10.1016/j.carres.2019.03.011
|
| PII |
S0008-6215(19)30056-4
|
| PMID |
30933787
|
| MeSH |
Carbohydrate Conformation
Enzyme Assays / methods*
Pentosyltransferases / genetics
Pentosyltransferases / metabolism*
Uridine Diphosphate Sugars / chemical synthesis*
Uridine Diphosphate Sugars / chemistry
Uridine Diphosphate Sugars / metabolism*
|
| IF |
1.873
|
| Times Cited |
1
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda01652 |
