RRC ID 54611
Author Bourguet P, de Bossoreille S, López-González L, Pouch-Pélissier MN, Gómez-Zambrano Á, Devert A, Pélissier T, Pogorelcnik R, Vaillant I, Mathieu O.
Title A role for MED14 and UVH6 in heterochromatin transcription upon destabilization of silencing.
Journal Life Sci Alliance
Abstract Constitutive heterochromatin is associated with repressive epigenetic modifications of histones and DNA which silence transcription. Yet, particular mutations or environmental changes can destabilize heterochromatin-associated silencing without noticeable changes in repressive epigenetic marks. Factors allowing transcription in this nonpermissive chromatin context remain poorly known. Here, we show that the transcription factor IIH component UVH6 and the mediator subunit MED14 are both required for heat stress-induced transcriptional changes and release of heterochromatin transcriptional silencing in Arabidopsis thaliana. We find that MED14, but not UVH6, is required for transcription when heterochromatin silencing is destabilized in the absence of stress through mutating the MOM1 silencing factor. In this case, our results raise the possibility that transcription dependency over MED14 might require intact patterns of repressive epigenetic marks. We also uncover that MED14 regulates DNA methylation in non-CG contexts at a subset of RNA-directed DNA methylation target loci. These findings provide insight into the control of heterochromatin transcription upon silencing destabilization and identify MED14 as a regulator of DNA methylation.
Volume 1(6)
Pages e201800197
Published 2018-12-1
DOI 10.26508/lsa.201800197
PII LSA-2018-00197
PMID 30574575
PMC PMC6291795
IF 2.622
Times Cited 4
Resource
Arabidopsis / Cultured plant cells, genes pdz88624