RRC ID 54628
Author Tokuoka M, Kobayashi K, Satou Y.
Title Distinct regulation of Snail in two muscle lineages of the ascidian embryo achieves temporal coordination of muscle development.
Journal Development
Abstract The transcriptional repressor Snail is required for proper differentiation of the tail muscle of ascidian tadpole larvae. Two muscle lineages (B5.1 and B6.4) contribute to the anterior tail muscle cells, and are consecutively separated from a transcriptionally quiescent germ cell lineage at the 16- and 32-cell stages. Concomitantly, cells of these lineages begin to express Tbx6.b (Tbx6-r.b) at the 16- and 32-cell stages, respectively. Meanwhile, Snail expression begins in these two lineages simultaneously at the 32-cell stage. Here, we show that Snail expression is regulated differently between these two lineages. In the B5.1 lineage, Snail was activated through Tbx6.b, which is activated by maternal factors, including Zic-r.a. In the B6.4 lineage, the MAPK pathway was cell-autonomously activated by a constitutively active form of Raf, enabling Zic-r.a to activate Snail independently of Tbx6.b As a result, Snail begins to be expressed at the 32-cell stage simultaneously in these two lineages. Such shortcuts might be required for coordinating developmental programs in embryos in which cells become separated progressively from stem cells, including germline cells.
Volume 145(11)
Published 2018-6-6
DOI 10.1242/dev.163915
PII dev.163915
PMID 29764858
MeSH Animals Cell Differentiation / genetics Cell Differentiation / physiology Ciona intestinalis / embryology* Embryo, Nonmammalian / metabolism Fetal Proteins / biosynthesis Gene Expression Regulation, Developmental Larva / growth & development Muscle Development / genetics* Muscle Development / physiology Muscles / cytology Muscles / embryology* Snail Family Transcription Factors / genetics* T-Box Domain Proteins / biosynthesis
IF 5.763
Times Cited 2
C.intestinalis / (O.japonicus) Wild C. int