論文 - 詳細
| RRC ID | 54665 |
|---|---|
| 著者 | Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N. |
| タイトル | Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport. |
| ジャーナル | J Cell Biol |
| Abstract |
Kinesin-1, the founding member of the kinesin superfamily of proteins, is known to use only a subset of microtubules for transport in living cells. This biased use of microtubules is proposed as the guidance cue for polarized transport in neurons, but the underlying mechanisms are still poorly understood. Here, we report that kinesin-1 binding changes the microtubule lattice and promotes further kinesin-1 binding. This high-affinity state requires the binding of kinesin-1 in the nucleotide-free state. Microtubules return to the initial low-affinity state by washing out the binding kinesin-1 or by the binding of non-hydrolyzable ATP analogue AMPPNP to kinesin-1. X-ray fiber diffraction, fluorescence speckle microscopy, and second-harmonic generation microscopy, as well as cryo-EM, collectively demonstrated that the binding of nucleotide-free kinesin-1 to GDP microtubules changes the conformation of the GDP microtubule to a conformation resembling the GTP microtubule. |
| 巻・号 | 217(12) |
| ページ | 4164-4183 |
| 公開日 | 2018-12-3 |
| DOI | 10.1083/jcb.201711178 |
| PII | jcb.201711178 |
| PMID | 30297389 |
| PMC | PMC6279379 |
| MeSH | Adenylyl Imidodiphosphate / chemistry Adenylyl Imidodiphosphate / pharmacology Animals Biological Transport, Active Chlorocebus aethiops Dogs Guanosine Diphosphate / chemistry Guanosine Diphosphate / metabolism Guanosine Triphosphate / chemistry Guanosine Triphosphate / metabolism HeLa Cells Humans Kinesins* / chemistry Kinesins* / metabolism Madin Darby Canine Kidney Cells Microtubules* / chemistry Microtubules* / metabolism Vero Cells |
| IF | 8.891 |
| 引用数 | 17 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 47 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) HeLa(RCB0007) MDCK(RCB0995) |