論文 - 詳細
| RRC ID | 54677 |
|---|---|
| 著者 | Nagahama M, Takehara M, Kobayashi K. |
| タイトル | Interaction of Clostridium perfringens Iota Toxin and Lipolysis-Stimulated Lipoprotein Receptor (LSR). |
| ジャーナル | Toxins (Basel) |
| Abstract |
Iota toxin produced by Clostridium perfringens is a binary, actin ADP-ribosylating toxin that is organized into the enzymatically active component Ia and the binding component Ib. Lipolysis-stimulated lipoprotein receptor (LSR) has been identified as a cellular receptor of Ib. Here, we investigated the functional interaction between Ib and LSR, where siRNA for LSR blocked the toxin-mediated cytotoxicity and the binding of Ib. The addition of Ib to LSR-green fluorescence protein (GFP)-transfected cells at 4 °C resulted in colocalization with LSR and Ib on the cell surface. Upon transfer of the cells from 4 °C to 37 °C, LSR and Ib were internalized and observed in cytoplasmic vesicles. When the cells were incubated with Ib at 37 °C and fractionated using the Triton-insoluble membrane, Ib oligomer was localized in insoluble factions that fulfilled the criteria of lipid rafts, and LSR was clustered in lipid rafts. To examine the interaction between N-terminal extracellular region of LSR and Ib, we constructed a series of LSR N-terminal deletions. Ten amino acids residues can be deleted from this end without any reduction of Ib binding. However, deletion of 15 N-terminal residues drastically reduces its ability to bind Ib. These results demonstrate that Ib binds to the LSR N-terminal 10 to 15 residues and endocytoses into trafficking endosomes together with LSR. |
| 巻・号 | 10(10) |
| 公開日 | 2018-10-8 |
| DOI | 10.3390/toxins10100405 |
| PII | toxins10100405 |
| PMID | 30297616 |
| PMC | PMC6215307 |
| MeSH | A549 Cells ADP Ribose Transferases / genetics ADP Ribose Transferases / metabolism* Animals Bacterial Toxins / genetics Bacterial Toxins / metabolism* Dogs Humans Madin Darby Canine Kidney Cells RNA, Small Interfering / genetics Receptors, Lipoprotein / metabolism* |
| IF | 3.895 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) A549 |