RRC ID 54685
著者 Sano A, Shi H, Suzuki R, Shirataki Y, Sakagami H.
タイトル Change in Amino Acid Pools During Neuronal Differentiation of PC12 Cells.
ジャーナル In Vivo
Abstract BACKGROUND/AIM:Although rat PC12 cells are a well-established model to investigate neuronal differentiation, survival and function, the reports of differentiation-associated changes in the intracellular amino acid pools of neurotransmitters have been limited. In this study, possible changes in the intracellular amino acid pools were investigated during nerve growth factor (NGF)-induced differentiation of PC12 cells.
MATERIALS AND METHODS:Rat PC12 cells were induced to differentiate into neuronal cells by 50 ng/ml NGF in serum-free Dulbecco's Modified Eagle's medium, followed by the addition of fresh NGF-containing medium at day 3, without medium change. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Intracellular amino acids were extracted by 5%trichloroacetic acid and quantified by amino acid analyzer.
RESULTS:Differentiated PC12 cells showed high concentrations of excitatory neurotransmitters (glutamic acid and aspartic acid) and glutamine (energy supply). In contrast, urea and taurine levels declined with the progression of neuronal differentiation. Exogenous addition of taurine, urea, and L- and D- aspartic acid showed little or no effect on supporting viability of PC12 cells cultured in serum-free medium.
CONCLUSION:The present study demonstrated dramatic changes in the composition of intracellular amino acids during neuronal differentiation.
巻・号 32(6)
ページ 1403-1408
公開日 2018-1-1
DOI 10.21873/invivo.11392
PII 32/6/1403
PMID 30348694
PMC PMC6365737
MeSH Amino Acids / metabolism* Animals Aspartic Acid / pharmacology Biomarkers Cell Differentiation* / drug effects Cell Survival / drug effects Humans Nerve Growth Factor / metabolism Nerve Growth Factor / pharmacology Neurons / cytology* Neurons / drug effects Neurons / metabolism* PC12 Cells Rats Taurine / pharmacology Urea / pharmacology
IF 1.609
引用数 0
リソース情報
ヒト・動物細胞 PC-12(RCB0009)