論文 - 詳細
| RRC ID | 54700 |
|---|---|
| 著者 | Tanaka M, Ishige A, Yaguchi M, Matsumoto T, Shirouzu M, Yokoyama S, Ishikawa F, Kitabayashi I, Takemori T, Harada M. |
| タイトル | Development of a simple new flow cytometric antibody-dependent cellular cytotoxicity (ADCC) assay with excellent sensitivity. |
| ジャーナル | J Immunol Methods |
| Abstract |
Antibody-based therapeutic strategies have become recognized as useful clinical options in several types of cancer, often with the expectation that such therapies will trigger target cell elimination via antibody-dependent cellar cytotoxicity (ADCC) by natural killer cells. The successful development of therapeutic monoclonal antibodies (mAbs) requires an assay system that permits a critical evaluation of their physicochemical and biological characteristics. At present a number of ADCC assay systems have been reported, however, there is still room for improvement in terms of usability, operability and sensitivity. Here we report a novel flow cytometric ADCC assay that uses a human natural killer cell line stably transfected with mouse FcγRIII, and Fc receptor common-γ chain (FcRγ) and a reporter gene as effector cells. This assay relies on discriminating effector and target cells by their differential immunofluorescence, which allows for clear-cut gating and accurate calculation of the number of surviving cells in a target population. This assay is easy and quick to perform and provides reliable data even for low frequency target cells in assay samples and with low concentrations of mAbs. Furthermore, our approach allows us to identify synergistic ADCC activity of mAbs with different epitope specificities on the same target antigen. |
| 巻・号 | 464 |
| ページ | 74-86 |
| 公開日 | 2019-1-1 |
| DOI | 10.1016/j.jim.2018.10.014 |
| PII | S0022-1759(18)30276-X |
| PMID | 30389576 |
| MeSH | Animals Antibodies, Monoclonal / pharmacology* Antibody-Dependent Cell Cytotoxicity / drug effects* Antineoplastic Agents, Immunological / pharmacology* Cell Line, Tumor Cell Survival / drug effects Drug Resistance, Neoplasm Flow Cytometry / methods* Fluorescent Antibody Technique Humans Killer Cells, Natural / drug effects* Killer Cells, Natural / immunology Leukemia / drug therapy* Leukemia / genetics Leukemia / immunology Leukemia / pathology Mice, Inbred BALB C Mice, SCID Receptors, IgG / genetics Receptors, IgG / immunology |
| IF | 1.913 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A20(RCB2745) |