RRC ID 54704
Author Sun S, Li K, Xiao L, Lei Z, Zhang Z.
Title Characterization of polysaccharide from Helicteres angustifolia L. and its immunomodulatory activities on macrophages RAW264.7.
Journal Biomed Pharmacother
Abstract Helicteres angustifolia L. (H. angustifolia) has been widely used as a remedy against various types of illness relating to immune response, such as inflammations and fever. In order to characterize the structure and identify the immunomodulatory activity of polysaccharide from H. angustifolia, a polysaccharide fraction (SPF3-1) was purified from H. angustifolia by using DEAE Sepharose Fast Flow and Sephacry S-400 chromatography, successively. Physicochemical analysis demonstrated that SPF3-1 is an acidic heteropolysaccharide with a molecular weight of about 13.36 kDa; in vitro immunomodulatory assay reflects that SPF3-1 could significantly (p <  0.05) enhance the proliferation of macrophages, stimulate the macrophages phagocytic capacity, as well as induce NO and immunomodulatory cytokines generation. All the results suggest that SPF3-1 from H. angustifolia possesses potent immunomodulatory activity and could be further developed as new products for medicines or functional foods.
Volume 109
Pages 262-270
Published 2019-1
DOI 10.1016/j.biopha.2018.10.039
PII S0753-3322(18)35208-9
PMID 30396084
MeSH Animals Cell Line, Tumor Cell Survival / drug effects Cell Survival / immunology Dose-Response Relationship, Drug Humans Immunologic Factors / isolation & purification Immunologic Factors / pharmacology* Macrophages / drug effects* Macrophages / immunology Malvaceae* Mice Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plant Roots* Polysaccharides / isolation & purification Polysaccharides / pharmacology* RAW 264.7 Cells
IF 3.743
Times Cited 11
Human and Animal Cells RAW 264(RCB0535)