論文 - 詳細
| RRC ID | 54732 |
|---|---|
| 著者 | Mie M, Matsumoto R, Mashimo Y, Cass AEG, Kobatake E. |
| タイトル | Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting. |
| ジャーナル | Mol Biol Rep |
| Abstract |
Modification of protein-based drug carriers with tumor-targeting properties is an important area of research in the field of anticancer drug delivery. To this end, we developed nanoparticles comprised of elastin-like polypeptides (ELPs) with fused poly-aspartic acid chains (ELP-D) displaying DNA aptamers. DNA aptamers were enzymatically conjugated to the surface of the nanoparticles via genetic incorporation of Gene A* protein into the sequence of the ELP-D fusion protein. Gene A* protein, derived from bacteriophage ϕX174, can form covalent complexes with single-stranded DNA via the latter's recognition sequence. Gene A* protein-displaying nanoparticles exhibited the ability to deliver the anticancer drug paclitaxel (PTX), whilst retaining activity of the conjugated Gene A* protein. PTX-loaded protein nanoparticles displaying DNA aptamers known to bind to the MUC1 tumor marker resulted in increased cytotoxicity with MCF-7 breast cancer cells compared to PTX-loaded protein nanoparticles without the DNA aptamer modification. |
| 巻・号 | 46(1) |
| ページ | 261-269 |
| 公開日 | 2019-2-1 |
| DOI | 10.1007/s11033-018-4467-2 |
| PII | 10.1007/s11033-018-4467-2 |
| PMID | 30421127 |
| MeSH | Antineoplastic Agents / therapeutic use Aptamers, Nucleotide / therapeutic use Breast Neoplasms / drug therapy Cell Line, Tumor Drug Carriers / chemistry Drug Delivery Systems / methods* Elastin / administration & dosage* Elastin / metabolism Female Humans MCF-7 Cells Nanoparticles / therapeutic use* Neoplasms / drug therapy Paclitaxel / pharmacology |
| IF | 2.107 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MCF7(RCB1904) |