| RRC ID |
54732
|
| 著者 |
Mie M, Matsumoto R, Mashimo Y, Cass AEG, Kobatake E.
|
| タイトル |
Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting.
|
| ジャーナル |
Mol Biol Rep
|
| Abstract |
Modification of protein-based drug carriers with tumor-targeting properties is an important area of research in the field of anticancer drug delivery. To this end, we developed nanoparticles comprised of elastin-like polypeptides (ELPs) with fused poly-aspartic acid chains (ELP-D) displaying DNA aptamers. DNA aptamers were enzymatically conjugated to the surface of the nanoparticles via genetic incorporation of Gene A* protein into the sequence of the ELP-D fusion protein. Gene A* protein, derived from bacteriophage ϕX174, can form covalent complexes with single-stranded DNA via the latter's recognition sequence. Gene A* protein-displaying nanoparticles exhibited the ability to deliver the anticancer drug paclitaxel (PTX), whilst retaining activity of the conjugated Gene A* protein. PTX-loaded protein nanoparticles displaying DNA aptamers known to bind to the MUC1 tumor marker resulted in increased cytotoxicity with MCF-7 breast cancer cells compared to PTX-loaded protein nanoparticles without the DNA aptamer modification.
|
| 巻・号 |
46(1)
|
| ページ |
261-269
|
| 公開日 |
2019-2-1
|
| DOI |
10.1007/s11033-018-4467-2
|
| PII |
10.1007/s11033-018-4467-2
|
| PMID |
30421127
|
| MeSH |
Antineoplastic Agents / therapeutic use
Aptamers, Nucleotide / therapeutic use
Breast Neoplasms / drug therapy
Cell Line, Tumor
Drug Carriers / chemistry
Drug Delivery Systems / methods*
Elastin / administration & dosage*
Elastin / metabolism
Female
Humans
MCF-7 Cells
Nanoparticles / therapeutic use*
Neoplasms / drug therapy
Paclitaxel / pharmacology
|
| IF |
2.107
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
MCF7(RCB1904) |
