RRC ID 54748
Author Yamaguchi-Sekino S, Kira T, Sekino M, Akahane M.
Title Effects of 7 T static magnetic fields on the expression of biological markers and the formation of bone in rats.
Journal Bioelectromagnetics
Abstract In this study, we aimed to evaluate the effects of 7 T static magnetic fields (SMFs) on rat mesenchymal stem cells (MSCs) in order to determine whether strong SMFs affected the osteogenesis of MSCs. MSCs were prepared from bone marrow cells obtained from the femurs of 7-week-old male Fischer 344 rats. MSCs were then combined with β-tricalcium phosphate (β-TCP), yielding two types of TCP/MSC constructs (TCP/P-1 and P-2) on day 0. Exposure was performed for 3 h/day for 6 days, and the experiments were performed twice using different exposure apparatus (cryovials or 4-well chambers) for each experiment. The results from gene expression, protein expression, and histological analyses showed no reproducible effects on both TCP/P-1 and TCP/P-2 MSC constructs, although osteocalcin levels for TCP/P-1 MSC constructs increased significantly once after 7 T exposure in two experiments. These findings contribute to understanding the effects of strong SMFs on MSC and osteoblasts. Bioelectromagnetics. 40:16-26, 2019. © 2018 Wiley Periodicals, Inc.
Volume 40(1)
Pages 16-26
Published 2019-1
DOI 10.1002/bem.22161
PMID 30466173
MeSH Alkaline Phosphatase / genetics Animals Biomarkers / metabolism Cell Differentiation Femur / cytology Femur / metabolism Femur / physiology* Gene Expression Regulation* Magnetic Fields* Male Mesenchymal Stem Cells / cytology Osteocalcin / genetics Osteogenesis* Rats
IF 1.945
Times Cited 3
Human and Animal Cells B16/BL6(RCB2638)