論文 - 詳細
| RRC ID | 54760 |
|---|---|
| 著者 | Ishikawa K, Hara T, Kato K, Shimomura T, Omori K. |
| タイトル | Involvement of natriuretic peptide system in C2C12 myocytes. |
| ジャーナル | Mol Cell Biochem |
| Abstract |
The natriuretic peptide system, a key regulator of cGMP signaling, comprises three types of natriuretic peptides, osteocrin/musclin (OSTN), and their natriuretic peptide receptors. Although this system plays important roles in many organs, its physiological roles in skeletal muscle have not been clearly described. In the present study, we investigated the role of the natriuretic peptide system in C2C12 myocytes. All three natriuretic peptide receptors were expressed by cells differentiating from myoblasts to myotubes, and natriuretic peptide receptor B (NPR-B) transcripts were detected at the highest levels. Further, higher levels of cGMP were generated in response to stimulation with C-type natriuretic peptide (CNP) versus atrial natriuretic peptide (ANP), which reflected receptor expression levels. A cGMP analog downregulated the expression of a few ER stress-related genes. Furthermore, OSTN gene expression was strongly upregulated after 20 days of differentiation. Augmented gene expression was found to correlate closely with endoplasmic reticulum (ER) stress, and C/EBP [CCAAT-enhancer-binding protein] homologous protein (CHOP), which is known to be activated by ER stress, affected the expression of OSTN. Together, these results suggest a role for natriuretic peptide signaling in the ER stress response of myocytes. |
| 巻・号 | 456(1-2) |
| ページ | 15-27 |
| 公開日 | 2019-6-1 |
| DOI | 10.1007/s11010-018-3486-6 |
| PII | 10.1007/s11010-018-3486-6 |
| PMID | 30519782 |
| MeSH | Animals Cell Line Cyclic GMP / metabolism* Cyclic GMP / pharmacology Endoplasmic Reticulum Stress / drug effects* Gene Expression Regulation / drug effects Mice Muscle Fibers, Skeletal / metabolism* Muscle Proteins / biosynthesis Natriuretic Peptides / pharmacology* Second Messenger Systems / drug effects* |
| IF | 2.884 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) 293T(RCB2202) |