RRC ID 54760
Author Ishikawa K, Hara T, Kato K, Shimomura T, Omori K.
Title Involvement of natriuretic peptide system in C2C12 myocytes.
Journal Mol Cell Biochem
Abstract The natriuretic peptide system, a key regulator of cGMP signaling, comprises three types of natriuretic peptides, osteocrin/musclin (OSTN), and their natriuretic peptide receptors. Although this system plays important roles in many organs, its physiological roles in skeletal muscle have not been clearly described. In the present study, we investigated the role of the natriuretic peptide system in C2C12 myocytes. All three natriuretic peptide receptors were expressed by cells differentiating from myoblasts to myotubes, and natriuretic peptide receptor B (NPR-B) transcripts were detected at the highest levels. Further, higher levels of cGMP were generated in response to stimulation with C-type natriuretic peptide (CNP) versus atrial natriuretic peptide (ANP), which reflected receptor expression levels. A cGMP analog downregulated the expression of a few ER stress-related genes. Furthermore, OSTN gene expression was strongly upregulated after 20 days of differentiation. Augmented gene expression was found to correlate closely with endoplasmic reticulum (ER) stress, and C/EBP [CCAAT-enhancer-binding protein] homologous protein (CHOP), which is known to be activated by ER stress, affected the expression of OSTN. Together, these results suggest a role for natriuretic peptide signaling in the ER stress response of myocytes.
Volume 456(1-2)
Pages 15-27
Published 2019-6-1
DOI 10.1007/s11010-018-3486-6
PII 10.1007/s11010-018-3486-6
PMID 30519782
IF 2.884
Times Cited 2
Human and Animal Cells C2C12(RCB0987) 293T(RCB2202)