論文 - 詳細
| RRC ID | 54795 |
|---|---|
| 著者 | Islam Z, Inui T, Ishibashi O. |
| タイトル | Gpr137b is an orphan G-protein-coupled receptor associated with M2 macrophage polarization. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Macrophages are classified mainly into two subtypes, M1 and M2, which exhibit distinct phenotypes, based on their microenvironment. Although recent studies have suggested that G-protein-coupled receptors (GPCRs) are associated with M1/M2 macrophage polarization, available information on GPCR-mediated macrophage polarization is still limited. In the present study, we identified Gpr137b as an orphan GPCR abundantly expressed in RAW264, a mouse macrophage cell line, and illuminated its role in M2 macrophage polarization. We generated Gpr137b-knockout (Gpr137b-KO) clones of RAW264 cells using the CRISPR/Cas9 genome editing system. Two independent Gpr137b-KO clones were isolated, which were demonstrated to have frameshifting 188-nucleotide deletions at a region containing the ATG start codon of Gpr137b. Consistently, qRT-PCR analysis revealed that the deleted region is not transcribed. We then treated the Gpr137b-KO and wildtype RAW264 cells with interleukin-4 (IL-4) to induce M2 macrophage polarization. Microarray analysis revealed that the IL-4-induced gene expression of representative M2 macrophage markers was significantly reduced in the Gpr137b-KO cells, and this was validated by qRT-PCR analysis. By contrast, M1 macrophage marker gene expression induced by lipopolysaccharide was unaffected by Gpr137b-KO. Collectively, the current study shows that Gpr137b is a possible regulator of M2 macrophage polarization. |
| 巻・号 | 509(3) |
| ページ | 657-663 |
| 公開日 | 2019-2-12 |
| DOI | 10.1016/j.bbrc.2018.12.140 |
| PII | S0006-291X(18)32805-5 |
| PMID | 30595385 |
| MeSH | Animals CRISPR-Cas Systems Cell Polarity Gene Expression Interleukin-4 / immunology* Lipopolysaccharides / immunology* Macrophages / cytology Macrophages / immunology* Macrophages / metabolism Mice RAW 264.7 Cells Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / immunology* |
| IF | 2.705 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |