| RRC ID |
54805
|
| 著者 |
Kawasaki T, Sato A, Tominaga Y, Suzuki Y, Oyama T, Tadokoro M, Tsukiyama K, Nokihara K, Zen H.
|
| タイトル |
Photo-Modification of Melanin by a Mid-infrared Free-electron Laser.
|
| ジャーナル |
Photochem Photobiol
|
| Abstract |
Melanin is rigidly constructed by several nitrogen-containing aromatic rings, and its excess accumulation in skin tissue is closely associated with melanosis. Although visible lasers (wavelength: 600-1000 nm) are conventionally used for the photo-thermolysis of melanocyte, several pigmented nevi are difficult to be treated. Here, we propose an alternate method for targeting the molecular structure of melanin using an infrared free-electron laser (FEL) tuned to 5.8 μm that corresponds to the stretching vibrational mode of carboxylate group. A drastic morphological change on the black-colored surface of melanin powder was observed after the pulse irradiation with power energy of 500 mJ cm-2 , and the minimum irradiation time for damage to the morphology was 1.4 s. Analyses by mass spectroscopy, infrared spectroscopy, and 13 C-nuclear magnetic resonance implied that a pyrrole group was removed by the FEL irradiation. In addition, the FEL irradiation dispersed almost all of the melanoma cells from a culture solution without any influence on other ingredients in the medium, and one-cell analysis by infrared microscopy showed that the structure of melanoma could be substantially damaged by the irradiation. This study proposes the potency of intense mid-infrared laser as novel alternative way to reduce melanin.
|
| 巻・号 |
95(4)
|
| ページ |
946-950
|
| 公開日 |
2019-7-1
|
| DOI |
10.1111/php.13079
|
| PMID |
30613988
|
| MeSH |
Cell Line, Tumor
Humans
Laser Therapy
Lasers / classification*
Melanins / chemistry*
Melanoma / radiotherapy
Microscopy
|
| IF |
2.338
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma 4A5(RCB0557) |
