| RRC ID |
54811
|
| 著者 |
Wang W, Nakashima S, Nakamura S, Oda Y, Matsuda H.
|
| タイトル |
Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42-Rac1-RhoA signaling protein levels.
|
| ジャーナル |
J Nat Med
|
| Abstract |
Auriculataoside A, an anthracenone dimer glycoside isolated from Cassia auriculata seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC50 value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action underlying the anti-proliferative effect of auriculataoside A on cells, we examined changes in whole protein expression after treatment with auriculataoside A and found that the expression Cdc42, RhoA, and Rac1, which are Rho family GTPases, was reduced. Auriculataoside A also arrested the cell cycle at G1 phase. These results suggest that the suppression of the above proteins induced G1 arrest. In addition, auriculataoside A also suppressed the expression of β-catenin and c-Myc proteins. This action of auriculataoside A could be one of the mechanisms underlying its selective anti-proliferative effect on B16 melanoma cells.
|
| 巻・号 |
73(3)
|
| ページ |
450-455
|
| 公開日 |
2019-6-1
|
| DOI |
10.1007/s11418-018-01278-0
|
| PII |
10.1007/s11418-018-01278-0
|
| PMID |
30627935
|
| MeSH |
Animals
Cell Line, Tumor
Humans
Melanoma, Experimental / drug therapy*
Melanoma, Experimental / pathology
cdc42 GTP-Binding Protein / metabolism*
rhoA GTP-Binding Protein / metabolism*
|
| IF |
1.966
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma 4A5(RCB0557) |
