| RRC ID |
54820
|
| 著者 |
Kodama Y, Harinath D, Mihara-Tomiyama N, Tominaga N, Ide Y, Nakahara T, Maeda M, Igarashi M, D'Armiento J, Chada K, Imai K.
|
| タイトル |
Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Tooth formation is accomplished under strict genetic programs. Although patients with chromosome 12q14 aberration shows tooth phenotype including the size and eruption timing with bone growth anomaly, its etiology is uncertain. Here, we examined expression of Hmga2, which is encoded at chromosome 12q14, in mouse tooth germs and analyzed the involvement in lower first molar (M1) and mandibular bone development. Hmga2 expression was immunohistochemically detected at enamel organ and the surrounding mesenchyme of the M1 germs. The expression was dynamically changed with gestation and rapidly decreased in postnatal mice. In Hmga2-/- mice, the M1 germs and crowns were diminished in size, and formation and eruption of molars were delayed with mandibular bone growth retardation. Hmga2 cDNA or siRNA transfection showed that Hmga2 transcriptionally up-regulates expression of stem cell factors, Sox2 and Nanog. They were co-localized with Hmga2 in the germs, but differentially distributed at enamel organ and mesenchyme in Hmga2-/- mice. These results demonstrate that Hmga2 expressed in tooth germs regulates the growth, sizing and eruption and stem cell factor expression in different compartment of the germ and associates with mandibular bone growth. Although future studies are needed, the present study demonstrates HMGA2 regulation of tooth genesis with skeletal development.
|
| 巻・号 |
509(4)
|
| ページ |
1008-1014
|
| 公開日 |
2019-2-19
|
| DOI |
10.1016/j.bbrc.2019.01.017
|
| PII |
S0006-291X(19)30023-3
|
| PMID |
30654938
|
| MeSH |
Animals
Gene Expression Regulation, Developmental
HMGA2 Protein / analysis
HMGA2 Protein / metabolism
HMGA2 Protein / physiology*
Immunohistochemistry
Mandible / growth & development
Mice
Molar / growth & development
Nanog Homeobox Protein / metabolism*
Odontogenesis / drug effects
SOXB1 Transcription Factors / metabolism*
|
| IF |
2.705
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
3T3 Swiss Albino(RCB1642) |
