Reference - Detail
| RRC ID | 54828 |
|---|---|
| Author | Nishimura T, Nakauchi H. |
| Title | Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cells. |
| Journal | Methods Mol Biol |
| Abstract |
Human induced pluripotent stem cells (iPSCs) are a potential source of blood cells for transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. The development of widely varying reprogramming methods has enabled us nowadays to obtain iPSCs even from a small number of antigen-specific T cells from patients. As these T-cell-derived iPSCs (T-iPSCs) carry TCR gene rearrangements in their genomic DNA, they are likely useful for producing antigen-specific T cells and for studying T-cell development. T-cell immunotherapy is potentially an effective therapeutic strategy against many types of cancers and viral infections. If antigen-specific T cells tailored against diseases and for patients can be easily obtained, T-cell immunotherapy should become a popular choice of therapy. Here, we show the in vitro way to guide T-iPSCs sequentially to yield hematopoietic stem/progenitor cells (HSPCs), T-lineage cells, and mature CD8 single-positive T cells. These in vitro-generated CD8+ T cells display antigen-specific cytotoxity and perform general T-cell functions. This novel protocol thus provides means to generate antigen-specific T cells as well as chances to study normal human lymphopoiesis. It may help identify, and then clear away, barriers to T-cell immunotherapy such as immunological tolerance and cell exhaustion. T-iPSCs can confer their juvenile status upon their descendant T cells during pluripotency reprogramming and redifferentiation. This phenomenon should help to eliminate T-cell exhaustion. |
| Volume | 1899 |
| Pages | 25-40 |
| Published | 2019-1-1 |
| DOI | 10.1007/978-1-4939-8938-6_3 |
| PMID | 30649763 |
| MeSH | CD8 Antigens / immunology* Cell Culture Techniques / methods* Humans Immunotherapy Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / immunology Receptors, Antigen, T-Cell / genetics Receptors, Antigen, T-Cell / immunology T-Lymphocytes / cytology* T-Lymphocytes / immunology |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | OP9/N-DLL1(RCB2927) |