論文 - 詳細
| RRC ID | 54863 |
|---|---|
| 著者 | Nishimura K, Ishiwata H, Sakuragi Y, Hayashi Y, Fukuda A, Hisatake K. |
| タイトル | Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells. |
| ジャーナル | Sci Rep |
| Abstract |
Pluripotent stem cells (PSCs) have various degrees of pluripotency, which necessitates selection of PSCs with high pluripotency before their application to regenerative medicine. However, the quality control processes for PSCs are costly and time-consuming, and it is essential to develop inexpensive and less laborious selection methods for translation of PSCs into clinical applications. Here we developed an imaging system, termed Phase Distribution (PD) imaging system, which visualizes subcellular structures quantitatively in unstained and unlabeled cells. The PD image and its derived PD index reflected the mitochondrial content, enabling quantitative evaluation of the degrees of somatic cell reprogramming and PSC differentiation. Moreover, the PD index allowed unbiased grouping of PSC colonies into those with high or low pluripotency without the aid of invasive methods. Finally, the PD imaging system produced three-dimensional images of PSC colonies, providing further criteria to evaluate pluripotency of PSCs. Thus, the PD imaging system may be utilized for screening of live PSCs with potentially high pluripotency prior to more rigorous quality control processes. |
| 巻・号 | 9(1) |
| ページ | 1777 |
| 公開日 | 2019-2-11 |
| DOI | 10.1038/s41598-018-37779-x |
| PII | 10.1038/s41598-018-37779-x |
| PMID | 30741960 |
| PMC | PMC6370783 |
| MeSH | Animals Cell Differentiation Fluorescent Dyes Humans Mice Microscopy, Fluorescence / methods* Mitochondria / ultrastructure NIH 3T3 Cells Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / ultrastructure Subcellular Fractions / ultrastructure* |
| IF | 4.011 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | EB5(AES0151) |