論文 - 詳細
| RRC ID | 54866 |
|---|---|
| 著者 | Dion J, Minoshima F, Saito S, Kiyoi K, Hasehira K, Tateno H. |
| タイトル | Photoactivable Elimination of Tumorigenic Human Induced Pluripotent Stem Cells by Using a Lectin-Doxorubicin Prodrug Conjugate. |
| ジャーナル | Chembiochem |
| Abstract |
Human pluripotent stem cells (hPSCs) are attractive resources for regenerative medicine, but medical applications are hindered by their tumorigenic potential. Previously, a hPSC-specific lectin probe, rBC2LCN, was identified through comprehensive glycome analysis by using high-density lectin microarrays. Herein, a lectin-doxorubicin (DOX) prodrug conjugate, with controllable photolysis activation for the elimination of tumorigenic human induced pluripotent stem cells, has been developed. rBC2LCN was fused with a biotin-binding protein, tamavidin (BC2Tama), and the fusion protein was expressed in Escherichia coli and purified by means of affinity chromatography. BC2Tama was then conjugated with doxorubicin-photocleavable biotin (DOXPCB). The BC2Tama-DOXPCB conjugates were observed to bind to hPSCs followed by internalization. Upon exposure to ultraviolet light, DOX was released inside the cells, which allowed specific killing of the hPSCs. Thus, BC2Tama-DOXPCB should be useful for the targeted elimination of hPSCs contained in hPSC-derived cell therapy products. This is the first report of the generation of lectin-prodrug conjugates. BC2Tama should be applicable for the targeted delivery of various types of biotinylated compounds into hPSCs. |
| 巻・号 | 20(12) |
| ページ | 1606-1611 |
| 公開日 | 2019-6-14 |
| DOI | 10.1002/cbic.201900086 |
| PMID | 30737871 |
| MeSH | Biotin / chemistry Carcinogenesis / drug effects Cell Survival / drug effects Cloning, Molecular Doxorubicin / chemistry Doxorubicin / pharmacology* Escherichia coli / genetics Humans Induced Pluripotent Stem Cells / cytology* Lectins / chemistry Lectins / pharmacology* Prodrugs |
| IF | 2.641 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 253G1(HPS0002) |