RRC ID 54890
Author Miyake S, Ogo A, Kubota H, Teramoto F, Hirai T.
Title β-Hydroxy-β-methylbutyrate Suppresses NF-ĸB Activation and IL-6 Production in TE-1 Cancer Cells.
Journal In Vivo
Abstract BACKGROUND/AIM:Stress reactions, especially those related to surgery, cause poor convalescence of cancer patients. β-Hydroxyβ-methylbutyrate (HMB) is known to regulate excessive inflammation in the body. The objective of this work was to investigate the capacity of HMB to suppress activation of nuclear factor-kappa B (NF-ĸB) and production of interleukin-6 (IL-6) in a human esophageal squamous cell carcinoma cell line (TE-1).
MATERIALS AND METHODS:Cell proliferation was measured using the water-soluble tetrazolium-1 method, while tumor necrosis factor alpha (TNFα)-induced IL-6 production was measured using an enzyme-linked immunosorbent assay (ELISA) assay. Nuclear translocation of NF-ĸB was detected by immunofluorescence staining.
RESULTS:HMB did not affect cell proliferation. However, HMB suppressed the TNFα-induced increase in IL-6 production in TE-1 cells by inhibiting NF-ĸB activation.
CONCLUSION:HMB did not influence TE-1 cell proliferation, but inhibited activation of NF-ĸB and IL-6 production. This result may be useful for improving excessive stress reactions during and after surgery.
Volume 33(2)
Pages 353-358
Published 2019-3-1
DOI 10.21873/invivo.11481
PII 33/2/353
PMID 30804112
PMC PMC6506311
MeSH Cell Line, Tumor Cell Proliferation / drug effects Esophageal Neoplasms / drug therapy* Esophageal Neoplasms / genetics Esophageal Neoplasms / pathology Esophageal Squamous Cell Carcinoma / drug therapy* Esophageal Squamous Cell Carcinoma / genetics Esophageal Squamous Cell Carcinoma / pathology Gene Expression Regulation, Neoplastic / genetics Humans Interleukin-6 / genetics* NF-kappa B / genetics Tumor Necrosis Factor-alpha / genetics Valerates / pharmacology*
IF 1.609
Times Cited 1
Resource
Human and Animal Cells TE-1(RCB1894)