論文 - 詳細
| RRC ID | 54890 |
|---|---|
| 著者 | Miyake S, Ogo A, Kubota H, Teramoto F, Hirai T. |
| タイトル | β-Hydroxy-β-methylbutyrate Suppresses NF-ĸB Activation and IL-6 Production in TE-1 Cancer Cells. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND/AIM:Stress reactions, especially those related to surgery, cause poor convalescence of cancer patients. β-Hydroxyβ-methylbutyrate (HMB) is known to regulate excessive inflammation in the body. The objective of this work was to investigate the capacity of HMB to suppress activation of nuclear factor-kappa B (NF-ĸB) and production of interleukin-6 (IL-6) in a human esophageal squamous cell carcinoma cell line (TE-1). MATERIALS AND METHODS:Cell proliferation was measured using the water-soluble tetrazolium-1 method, while tumor necrosis factor alpha (TNFα)-induced IL-6 production was measured using an enzyme-linked immunosorbent assay (ELISA) assay. Nuclear translocation of NF-ĸB was detected by immunofluorescence staining. RESULTS:HMB did not affect cell proliferation. However, HMB suppressed the TNFα-induced increase in IL-6 production in TE-1 cells by inhibiting NF-ĸB activation. CONCLUSION:HMB did not influence TE-1 cell proliferation, but inhibited activation of NF-ĸB and IL-6 production. This result may be useful for improving excessive stress reactions during and after surgery. |
| 巻・号 | 33(2) |
| ページ | 353-358 |
| 公開日 | 2019-1-1 |
| DOI | 10.21873/invivo.11481 |
| PII | 33/2/353 |
| PMID | 30804112 |
| PMC | PMC6506311 |
| MeSH | Cell Line, Tumor Cell Proliferation / drug effects Esophageal Neoplasms / drug therapy* Esophageal Neoplasms / genetics Esophageal Neoplasms / pathology Esophageal Squamous Cell Carcinoma / drug therapy* Esophageal Squamous Cell Carcinoma / genetics Esophageal Squamous Cell Carcinoma / pathology Gene Expression Regulation, Neoplastic / genetics Humans Interleukin-6 / genetics* NF-kappa B / genetics Tumor Necrosis Factor-alpha / genetics Valerates / pharmacology* |
| IF | 1.609 |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | TE-1(RCB1894) |