| RRC ID |
5490
|
| Author |
Hoshino T, Nakabayashi T, Hirota K, Matsuno T, Koiwa R, Fujiu S, Saito I, Tkachenko OB, Matsuyama H, Yumoto I.
|
| Title |
Paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two Gram-variable, aerobic, motile, rod-shaped, endospore-forming bacterial strains, M4-2T and M4-1, were isolated from soil samples collected from Oblast Magadan, Russian Far East, as micro-organisms antagonistic to the psychrophilic phytopathogenic fungus Typhula ishikariensis. Strains M4-2T and M4-1 were identified as members of the genus Paenibacillus by phenotypic and phylogenetic analyses based on 16S rRNA gene sequences. The strains contained anteiso-C15:0 as the major fatty acid (63.0-64.7%) and MK-7 as the major isoprenoid quinone. The DNA G+C contents were 42.8 and 41.7 mol%, respectively. 16S rRNA gene sequence analysis showed that strains M4-2T and M4-1 exhibited high similarities with Paenibacillus macquariensis DSM 2T (99.5 and 99.7%, respectively) and Paenibacillus antarcticus LMG 22078T (99.4 and 99.5%, respectively). There were no clear differences in the phenotypic characteristics and chemotaxonomic and phylogenetic data between the novel isolates and P. macquariensis DSM 2T. DNA-DNA hybridization experiments between strain M4-2T and P. macquariensis DSM 2T and P. antarcticus LMG 22078T revealed reassociation values of 56 and 49%, respectively. Multilocus sequence analysis confirmed the differences between the new isolates and reference strains that were observed with the DNA-DNA hybridization studies. On the basis of the results described above, it is proposed that the isolates represent a novel subspecies of P. macquariensis, Paenibacillus macquariensis subsp. defensor subsp. nov. The type strain is M4-2T (=JCM 14954T=NCIMB 14397T).
|
| Volume |
59(Pt 8)
|
| Pages |
2074-9
|
| Published |
2009-8-1
|
| DOI |
10.1099/ijs.0.006304-0
|
| PII |
ijs.0.006304-0
|
| PMID |
19605719
|
| MeSH |
Aerobiosis
Antibiosis
Asia, Eastern
Bacterial Typing Techniques
Base Composition
Basidiomycota / growth & development
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Gram-Positive Bacteria / classification*
Gram-Positive Bacteria / genetics
Gram-Positive Bacteria / isolation & purification*
Gram-Positive Bacteria / physiology
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Spores, Bacterial / cytology
|
| IF |
2.415
|
| Times Cited |
3
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM14954 |
