RRC ID 54922
著者 Kasuda S, Kudo R, Yuui K, Sakurai Y, Hatake K.
タイトル Induced Pluripotent Stem Cell-Derived Hematopoietic Embryoid Bodies Improve Mouse Status in Septic Peritonitis.
ジャーナル Bull Exp Biol Med
Abstract We examined the efficacy of embryoid bodies from 6-day induced pluripotent stem cells an in vivo sepsis model. Injection of embryoid bodies to septic mice improved the condition of their lungs and significantly increased their survival rate. Although embryoid bodies secretedsphingosine-1-phosphate in vitro, its serum levels in mouse plasma were significantly reduced compared to that in the control (untreated mice receiving PBS). Low concentrations of sphingosine-1-phosphate protected endothelial cells, while high concentrations disrupted endothelial barrier integrity. Therefore, exogenous sphingosine-1-phosphate secreted by embryoid bodies during early stage of sepsis might down regulate endogenous production of sphingosine-1-phosphate. Inhibition of excessive sphingosine-1-phosphate release protects against endothelial injury and suppresses a vicious cycle of inflammatory reactions. The obtained results open new prospects in induced pluripotent stem cells-based therapy for sepsis.
巻・号 166(5)
ページ 667-670
公開日 2019-3-1
DOI 10.1007/s10517-019-04414-2
PII 10.1007/s10517-019-04414-2
PMID 30903503
MeSH Animals Embryoid Bodies / cytology* Endothelial Cells / cytology Hematopoietic Stem Cell Transplantation Induced Pluripotent Stem Cells / cytology* Lysophospholipids / metabolism Male Mice Mice, Inbred C57BL Peritonitis / metabolism Peritonitis / therapy* Sepsis Sphingosine / analogs & derivatives Sphingosine / metabolism
IF 0.567
引用数 0
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)