RRC ID 54926
著者 Sritharan S, Kannan TP, Norazmi MN, Nurul AA.
タイトル The synergistic effects of IL-6/IL-17A promote osteogenic differentiation by improving OPG/RANKL ratio and adhesion of MC3T3-E1 cells on hydroxyapatite.
ジャーナル J Craniomaxillofac Surg
Abstract OBJECTIVE:In this study, we evaluated the potential role of IL-6 and/or IL-17A in regulating the OPG/RANKL (osteoprotegerin/receptor activator of nuclear factor kappa b ligand) system of murine osteoblast cell line (MC3T3-E1) cultured on hydroxyapatite (HA).
METHODS:MC3T3-E1 cells were seeded on HA and treated with recombinant IL-6 or rIL-17A or combination of the two cytokines. Cell proliferation and differentiation activity were measured by MTS and alkaline phosphatase assays respectively. Observation of cell adhesion and proliferation was examined by scanning electron microscopy. Gene and protein expressions were performed on RANKL and OPG using qPCR, Western blot and ELISA.
RESULTS:We demonstrated that treatment with recombinant IL-17A (rIL-17A) and the combination rIL-6/rIL-17A promoted better adhesion and higher proliferation of cells on HA. Cells treated with rIL-17A and the combination cytokines showed a significant increase in differentiation activity on day 7, 10 and 14 as indicated by ALP activity (p < 0.001). Gene and protein expressions showed significant up-regulation of OPG and ALP (p < 0.001) and down-regulation of RANKL (p < 0.001) expression by all the treated groups. Interestingly, the combination of the two cytokines resulted in a significant increase of OPG/RANKL ratio (p < 0.001).
CONCLUSION:These findings indicated that treatment with the combination of the two cytokines (IL-6/IL-17A) has synergistic effects to promote osteoblastic differentiation but suppress osteoclastogenesis by altering the OPG/RANKL ratio.
巻・号 46(8)
ページ 1361-1367
公開日 2018-8-1
DOI 10.1016/j.jcms.2018.05.002
PII S1010-5182(18)30206-3
PMID 29805067
MeSH Animals Cell Adhesion / drug effects Cell Differentiation / drug effects Cell Line Cell Proliferation / drug effects Drug Synergism Durapatite / metabolism* Interleukin-17 / administration & dosage Interleukin-17 / pharmacology* Interleukin-6 / administration & dosage Interleukin-6 / pharmacology* Mice Osseointegration / drug effects Osteoblasts / drug effects* Osteoblasts / metabolism Osteogenesis / drug effects* Osteoprotegerin / metabolism* RANK Ligand / metabolism*
IF 1.96
引用数 1
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)