| RRC ID |
54944
|
| 著者 |
Sadhukhan A, Kobayashi Y, Nakano Y, Iuchi S, Kobayashi M, Sahoo L, Koyama H.
|
| タイトル |
Genome-wide Association Study Reveals that the Aquaporin NIP1;1 Contributes to Variation in Hydrogen Peroxide Sensitivity in Arabidopsis thaliana.
|
| ジャーナル |
Mol Plant
|
| Abstract |
Hydrogen peroxide (H2O2) is a reactive oxygen species that affects cell signaling in various plant defense responses and induces programmed cell death. To identify the new components associated with H2O2 signaling and tolerance, we conducted a genome-wide association study (GWAS) on the root growth of 133 Arabidopsis thaliana accessions grown in the presence of toxic H2O2 levels. The most significant SNPs were associated with a cluster of chromosome 4 genes encoding an aquaporin NODULIN 26-LIKE INTRINSIC PROTEIN 1; 1 (NIP1;1), an NB-ARC domain-containing disease resistance protein (AT4G19050), and a putative membrane lipoprotein (AT4G19070). The expression level of NIP1;1 was relatively high in A. thaliana accessions sensitive to H2O2. Additionally, overexpression of NIP1;1 in a tolerant accession (e.g., Col-0) increased the sensitivity of transgenic plants to H2O2. An in planta β-glucuronidase reporter assay revealed that variations in the NIP1;1 promoter were responsible for the differences of its expression level in H2O2-tolerant and -sensitive accessions. Cell death was extensive and H2O2 levels were high in the roots of H2O2-sensitive and NIP1;1-overexpressing accessions. Together, our results indicate that the aquaporin NIP1;1 is a key determinant of the sensitivity of A. thaliana to H2O2, and contributes to the phenotypic variations detected by our GWAS.
|
| 巻・号 |
10(8)
|
| ページ |
1082-1094
|
| 公開日 |
2017-8-7
|
| DOI |
10.1016/j.molp.2017.07.003
|
| PII |
S1674-2052(17)30199-5
|
| PMID |
28712931
|
| MeSH |
Aquaporins / genetics
Aquaporins / metabolism
Arabidopsis / drug effects
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Gene Expression Regulation, Plant / drug effects
Genome-Wide Association Study / methods*
Hydrogen Peroxide / pharmacology*
Plant Roots / drug effects
Plant Roots / genetics
Plant Roots / metabolism
|
| IF |
10.812
|
| 引用数 |
10
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
sja |
