| RRC ID |
54950
|
| Author |
Kikuchi M, Nishimura T, Saito D, Shigenobu S, Takada R, Gutierrez-Triana JA, Cerdán JLM, Takada S, Wittbrodt J, Suyama M, Tanaka M.
|
| Title |
Novel components of germline sex determination acting downstream of foxl3 in medaka.
|
| Journal |
Dev Biol
|
| Abstract |
Germline sex determination is an essential process for the production of sexually dimorphic gametes. In medaka, Forkhead box L3 (foxl3) was previously identified as a germ cell-intrinsic regulator of sex determination that suppresses the initiation of spermatogenesis in female germ cells. To reveal the molecular mechanism of germline sex determination by foxl3, we conducted the following four analyses: Comparison of transcriptomes between wild-type and foxl3-mutant germ cells; epistatic analysis; identification of the FOXL3-binding motif; and ChIP-qPCR assay using a FOXL3-monoclonal antibody. We identified two candidate genes acting downstream of foxl3: Rec8a and fbxo47. It has been known that Rec8 regulates sister chromatid cohesion and Fbxo47 acts as a ubiquitin E3 ligase. These functions have not been, however, associated with sexual differentiation in germ cells. Our results uncover novel components acting downstream of foxl3, providing insights into the mechanism of germline sex determination.
|
| Volume |
445(1)
|
| Pages |
80-89
|
| Published |
2019-1-1
|
| DOI |
10.1016/j.ydbio.2018.10.019
|
| PII |
S0012-1606(18)30516-5
|
| PMID |
30392839
|
| MeSH |
Animals
Female
Forkhead Transcription Factors / genetics
Gene Expression Profiling / methods
Germ Cells
Gonads / cytology
Male
Oogenesis / physiology
Oryzias / embryology*
Oryzias / genetics
Sex Determination Processes / physiology*
Sex Differentiation / genetics*
Spermatogenesis / physiology
|
| IF |
2.896
|
| Times Cited |
3
|
| Resource |
| Medaka |
cDNA clones: foxl3 (olgi46a18), atp1a3a (olbr29a24), fbxo47 (olte61d07), ces2l (olvl64m02)
fosmid: GOLWFno345_a19 |
