| RRC ID |
54979
|
| Author |
Tabor KM, Marquart GD, Hurt C, Smith TS, Geoca AK, Bhandiwad AA, Subedi A, Sinclair JL, Rose HM, Polys NF, Burgess HA.
|
| Title |
Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping.
|
| Journal |
Elife
|
| Abstract |
Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
|
| Volume |
8
|
| Published |
2019-2-8
|
| DOI |
10.7554/eLife.42687
|
| PII |
42687
|
| PMID |
30735129
|
| PMC |
PMC6392497
|
| MeSH |
Animals
Animals, Genetically Modified / genetics
Brain / physiology
Brain / ultrastructure*
Brain Mapping / methods*
Cell Lineage / genetics
DNA-Binding Proteins / genetics
Gene Expression Regulation / genetics
Integrases / genetics
Neuroimaging / methods*
Neurons / physiology
Neurons / ultrastructure*
Transcription Factors / genetics
Zebrafish / genetics
Zebrafish / physiology
|
| IF |
7.551
|
| Times Cited |
6
|
| Resource |
| Zebrafish |
Tg(vglut2a:loxP-DsRed-loxP-GFP) |
