RRC ID 54997
Author Hiscock TW, Miesfeld JB, Mosaliganti KR, Link BA, Megason SG.
Title Feedback between tissue packing and neurogenesis in the zebrafish neural tube.
Journal Development
Abstract Balancing the rate of differentiation and proliferation in developing tissues is essential to produce organs of robust size and composition. Although many molecular regulators have been established, how these connect to physical and geometrical aspects of tissue architecture is poorly understood. Here, using high-resolution timelapse imaging, we find that changes to cell geometry associated with dense tissue packing play a significant role in regulating differentiation rate in the zebrafish neural tube. Specifically, progenitors that are displaced away from the apical surface due to crowding, tend to differentiate in a Notch-dependent manner. Using simulations we show that interplay between progenitor density, cell shape and changes in differentiation rate could naturally result in negative-feedback control on progenitor cell number. Given these results, we suggest a model whereby differentiation rate is regulated by density dependent effects on cell geometry to: (1) correct variability in cell number; and (2) balance the rates of proliferation and differentiation over development to 'fill' the available space.
Volume 145(9)
Published 2018-5-4
DOI 10.1242/dev.157040
PII dev.157040
PMID 29678815
PMC PMC5992593
MeSH Animals Cell Differentiation / physiology* Cell Proliferation / physiology* Neural Stem Cells / cytology Neural Stem Cells / metabolism* Neural Tube / cytology Neural Tube / embryology* Neurogenesis / physiology* Receptors, Notch / genetics Receptors, Notch / metabolism Zebrafish / embryology* Zebrafish / genetics Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 5.763
Times Cited 5
Zebrafish Tg (Huc:Kaede)