RRC ID 54999
Author Hu B, Gao Y, Davies L, Woo S, Topczewski J, Jessen JR, Lin F.
Title Glypican 4 and Mmp14 interact in regulating the migration of anterior endodermal cells by limiting extracellular matrix deposition.
Journal Development
Abstract During embryogenesis, the germ layers, including the endoderm, undergo convergence and extension movements to narrow and elongate the body plan. In zebrafish, the dorsal migration of endodermal cells during gastrulation is controlled by chemokine signaling, but little is known about how they migrate during segmentation. Here, we show that glypican 4 (Gpc4), a member of the heparin sulfate proteoglycan family, is required for efficient migration of anterior endodermal cells during early segmentation, regulating Rac activation to maintain polarized actin-rich lamellipodia. An endoderm transplantation assay showed that Gpc4 regulates endoderm migration in a non-cell-autonomous fashion. Further analyses revealed that the impaired endoderm migration in gpc4 mutants results from increases in the expression and assembly of fibronectin and laminin, major components of the extracellular matrix (ECM). Notably, we found that matrix metalloproteinase 14 (Mmp14a/b) is required for the control of ECM expression during endoderm migration, with Gpc4 acting through Mmp14a/b to limit ECM expression. Our results suggest that Gpc4 is crucial for generating the environment required for efficient migration of endodermal cells, uncovering a novel function of Gpc4 during development.
Volume 145(17)
Published 2018-8-28
DOI 10.1242/dev.163303
PII dev.163303
PMID 30082271
MeSH Animals Animals, Genetically Modified Body Patterning / genetics Body Patterning / physiology* Cell Movement / genetics Cell Movement / physiology* Endoderm / embryology* Extracellular Matrix / metabolism Fibronectins / metabolism Gastrulation / physiology Glypicans / genetics Glypicans / metabolism* Laminin / metabolism Matrix Metalloproteinase 14 / genetics Matrix Metalloproteinase 14 / metabolism* Pseudopodia / metabolism Zebrafish / embryology* rac GTP-Binding Proteins / metabolism
IF 5.763
Times Cited 3
Resource
Zebrafish Tg(sox17:EGFP)