| RRC ID |
5499
|
| Author |
Niederberger TD, Steven B, Charvet S, Barbier B, Whyte LG.
|
| Title |
Virgibacillus arcticus sp. nov., a moderately halophilic, endospore-forming bacterium from permafrost in the Canadian high Arctic.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A novel, moderately halophilic, endospore-forming bacterial strain, designated Hal 1T, was isolated from a permafrost core collected from the Canadian high Arctic. The temperature for growth of strain Hal 1T was 0-30 degrees C with no growth observed at either -5 or 37 degrees C (optimum growth at about 25 degrees C). Strain Hal 1T was able to grow at NaCl concentrations of 0-20% (w/v) and did not have an absolute NaCl requirement for growth; optimal growth was at 5% (w/v) NaCl. The level of 16S rRNA gene sequence similarity between strain Hal 1T and the type strains of Virgibacillus carmonensis and Virgibacillus necropolis was 98.2%; values with respect to the type strains of other recognized Virgibacillus species were below 96.0%. The DNA G+C content of strain Hal 1T was 38.2 mol%. Levels of DNA-DNA relatedness between strain Hal 1T and the type strains of V. carmonensis and V. necropolis were 14.0 and 21.0%, respectively. The major fatty acid of strain Hal 1T was anteiso-C15:0, consistent with species of the genus Virgibacillus. The cell-wall peptidoglycan of strain Hal 1T was type A1alpha and the major respiratory quinone was MK-7. On the basis of genotypic and physiological results, strain Hal 1T (=DSM 19574T=JCM 14839T) is proposed as the type strain of a novel species of the genus Virgibacillus, namely Virgibacillus arcticus sp. nov.
|
| Volume |
59(Pt 9)
|
| Pages |
2219-25
|
| Published |
2009-9-1
|
| DOI |
10.1099/ijs.0.002931-0
|
| PII |
ijs.0.002931-0
|
| PMID |
19605723
|
| MeSH |
Arctic Regions
Bacillaceae / chemistry
Bacillaceae / classification*
Bacillaceae / genetics
Bacillaceae / isolation & purification*
Bacterial Typing Techniques
Base Composition
Canada
Cell Wall / chemistry
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Molecular Sequence Data
Nucleic Acid Hybridization
Peptidoglycan / analysis
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / metabolism*
Soil Microbiology*
Spores, Bacterial / cytology*
Temperature
|
| IF |
2.415
|
| Times Cited |
24
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM14839 |
