Author Nakatsukasa E, Kashiwazaki N, Takizawa A, Shino M, Kitada K, Serikawa T, Hakamata Y, Kobayashi E, Takahashi R, Ueda M, Nakashima T, Nakagata N.
Title Cryopreservation of spermatozoa from closed colonies, and inbred, spontaneous mutant, and transgenic strains of rats.
Journal Comp Med
Abstract We attempted to cryopreserve spermatozoa from closed colonies (Jcl:SD and Jcl:Wistar), and inbred (BN/Crj, F3441 DuCrj, LEW/Crj, Long-Evans and WKY/NCrj), mutant (Zitter [WTC.ZI-zi] and Tremor [TRM]), transgenic (human A-transferase [A], and green fluorescent protein [GFP]) strains of rats. Rat epididymal spermatozoa suspended in cryopreservation solution (23% egg yolk, 8% lactose monohydrate, and 0.7% Equex Stm, pH 7.4, adjusted with 10% Tris [hydroxymethy] aminomethane) were frozen and stored at -196 degrees C. After thawing at 37 degrees C, the spermatozoa were instilled into the tip of each uterine horn of the recipients. A total of five recipient females for each strain were inseminated with cryopreserved spermatozoa, and normal live offspring of all strains (Jcl:SD: 11, Jcl:Wistar: 13, BN/Crj: 9, F344/DuCrj: 28, LEW/Crj: 4, Long-Evans: 6, WKY/NCrj: 8, TRM: 24, WTC.ZI-zi: 27, A: 30 and GFP: 20) were obtained.
Volume 53(6)
Pages 639-41
Published 2003-12-1
PMID 14727812
MeSH Animals Animals, Genetically Modified* Breeding Cryopreservation / methods* Female Fertility / physiology* Fetal Viability / physiology Insemination, Artificial Litter Size Male Pregnancy Pregnancy Outcome Rats Rats, Inbred Strains Rats, Mutant Strains* Semen Preservation Sperm Motility / physiology Spermatozoa*
IF 1.067
Times Cited 30
Rats TRM/Kyo(strainID=11) WTC.ZI-Atrnzi/Kyo(strainID=22)