| RRC ID |
55031
|
| Author |
Makino Y, Jensen NH, Yokota N, Rossner MJ, Akiyama H, Shirahige K, Okada Y.
|
| Title |
Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression.
|
| Journal |
Sci Rep
|
| Abstract |
Gonocyte-to-spermatogonia transition is a critical fate determination process to initiate sperm production throughout the lifecycle. However, the molecular dynamics of this process has not been fully elucidated mainly due to the asynchronized differentiation stages of neonatal germ cells. In this study, we employed single cell RNA sequencing analyses of P1.5-5.5 germ cells to clarify the temporal dynamics of gene expression during gonocyte-to-spermatogonia transition. The analyses identified transcriptional modules, one of which regulates spermatogonial gene network in neonatal germ cells. Among them, we identified Dec2, a bHLH-type transcription factor, as a transcriptional repressor for a spermatogonial differentiation factor Sohlh1. Deficiency of Dec2 in mice induces significant reduction of undifferentiated spermatogonia, and transplantation assay using Dec2-depleted cells also demonstrated the impaired efficiency of engraftment, suggesting its role in maintaining spermatogonial stem cells (SSCs). Collectively, this study revealed the intrinsic role of a new SSC factor Dec2, which protects germ cells from inadequate differentiation during neonatal testis development.
|
| Volume |
9(1)
|
| Pages |
6063
|
| Published |
2019-4-15
|
| DOI |
10.1038/s41598-019-42578-z
|
| PII |
10.1038/s41598-019-42578-z
|
| PMID |
30988352
|
| PMC |
PMC6465314
|
| MeSH |
Animals
Animals, Newborn
Basic Helix-Loop-Helix Transcription Factors / genetics*
Cell Differentiation / genetics
Gene Expression Regulation, Developmental*
Male
Mice
Mice, Transgenic
Single-Cell Analysis
Spermatogenesis / genetics*
Spermatogonia / physiology*
Stem Cells / physiology
Testis / cytology
Testis / growth & development
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
4.011
|
| Times Cited |
3
|
| Resource |
| DNA material |
pENTR4-H1 (RDB04395)
CS-RfA-EVBsd (RDB06090)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
| Human and Animal Cells |
293T(RCB2202) |
