Reference - Detail
| RRC ID | 55032 |
|---|---|
| Author | Katano I, Nishime C, Ito R, Kamisako T, Mizusawa T, Ka Y, Ogura T, Suemizu H, Kawakami Y, Ito M, Takahashi T. |
| Title | Long-term maintenance of peripheral blood derived human NK cells in a novel human IL-15- transgenic NOG mouse. |
| Journal | Sci Rep |
| Abstract |
We generated a novel mouse strain expressing transgenic human interleukin-15 (IL-15) using the severe immunodeficient NOD/Shi-scid-IL-2Rγ null (NOG) mouse genetic background (NOG-IL-15 Tg). Human natural killer (NK) cells, purified from the peripheral blood (hu-PB-NK) of normal healthy donors, proliferated when transferred into NOG-IL-15 Tg mice. In addition, the cell number increased, and the hu-PB-NK cells persisted for 3 months without signs of xenogeneic graft versus host diseases (xGVHD). These in vivo-expanded hu-PB-NK cells maintained the original expression patterns of various surface antigens, including NK receptors and killer cell immunoglobulin-like receptor (KIR) molecules. They also contained significant amounts of granzyme A and perforin. Inoculation of K562 leukemia cells into hu-PB-NK-transplanted NOG-IL-15 Tg mice resulted in significant suppression of tumor growth compared with non-transplanted mice. Furthermore, NOG-IL-15 Tg mice allowed for engraftment of in vitro-expanded NK cells prepared for clinical cell therapy. These cells exerted antibody-dependent cell-mediated cytotoxicity (ADCC) on Her2-positive gastric cancer cells in the presence of therapeutic anti-Her2 antibody, and subsequently suppressed tumor growth. Our results collectively suggest that the NOG-IL-15 Tg mice are a useful model for studying human NK biology and evaluating human NK cell-mediated in vivo cytotoxicity. |
| Volume | 7(1) |
| Pages | 17230 |
| Published | 2017-12-8 |
| DOI | 10.1038/s41598-017-17442-7 |
| PII | 10.1038/s41598-017-17442-7 |
| PMID | 29222435 |
| PMC | PMC5722902 |
| MeSH | Animals Humans Interleukin-15 / blood* Interleukin-15 / genetics* Killer Cells, Natural / cytology* Mice Mice, Transgenic |
| IF | 4.011 |
| Times Cited | 8 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 15 |
| Altmetric score changes over past 6months | 6.0 |
| Resource | |
| DNA material | hIL-15 cDNA (RDB01866) |