論文 - 詳細
| RRC ID | 55037 |
|---|---|
| 著者 | Fukuda T, Katayama M, Yoshizawa T, Eitsuka T, Mizukami H, Nakagawa K, Ito H, Komagata H, Song S, Roh S, Hoshino Y, Sato E, Hanada H, Nishimori K, Miyazawa T, Uchida T. |
| タイトル | Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of the simian vacuolating virus 40 large T fragment. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals. |
| 巻・号 | 76(7) |
| ページ | 1372-7 |
| 公開日 | 2012-1-1 |
| DOI | 10.1271/bbb.120155 |
| PII | DN/JST.JSTAGE/bbb/120155 |
| PMID | 22785463 |
| MeSH | Animals Antigens, Polyomavirus Transforming / genetics* Cell Line* Cell Proliferation Embryo, Mammalian Fibroblasts / cytology* Fibroblasts / metabolism Fibroblasts / virology Founder Effect Gene Expression Genetic Engineering Integrases / genetics Karyotype Karyotyping Simian virus 40 / genetics* Swine |
| IF | 1.297 |
| 引用数 | 12 |
| リソース情報 | |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) |