RRC ID 55056
Author Nishiyama K, Nagai A, Uribayashi K, Yamamoto Y, Mukai T, Okada N.
Title Two extracellular sialidases from Bifidobacterium bifidum promote the degradation of sialyl-oligosaccharides and support the growth of Bifidobacterium breve.
Journal Anaerobe
Abstract We investigated the roles of extracellular sialidases (SiaBb1 and SiaBb2) in cross-feeding between sialidase-carrying Bifidobacterium bifidum and sialic acid-utilizing Bifidobacterium breve. Using 6' sialyllactose (6'SL) as a carbon source, the number of wild-type B. bifidum cells increased while that of a siabb2-inactivated strain (Δsiabb2) did not. Coculture of these two strains in the presence of 6'SL resulted in similar increase in cell numbers. Coculture of wild-type B. bifidum, but not the Δsiabb2 strain, with sialic acid-utilizing Bifidobacterium breve, which cannot release sialic acids from carbohydrates, in the presence of 6'SL increased the number of B. breve cells. Moreover, when mucin was used as a carbon source, B. breve growth was increased in cocultures with B. bifidum wild-type and Δsiabb2 strains, suggesting that SiaBb1 may be involved. Additionally, B. breve cell numbers increased during cultivation with recombinant SiaBb1-and SiaBb2-treated mucin as the sole carbon source. These results indicated that B. bifidum SiaBb2 liberated sialic acid from sialyl-human milk oligosaccharides and -mucin glycans, supporting the growth of B. breve through sialic acid cross-feeding. SiaBb1 may assist in the degradation of mucin glycan. Collectively, our results revealed that both the B. bifidum extracellular sialidases promote the utilization of sialylated carbohydrates and supply free sialic acid to other Bifidobacterium strains.
Volume 52
Pages 22-28
Published 2018-8-1
DOI 10.1016/j.anaerobe.2018.05.007
PII S1075-9964(18)30089-1
PMID 29787815
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism* Bifidobacterium bifidum / enzymology* Bifidobacterium bifidum / genetics Bifidobacterium breve / growth & development* Bifidobacterium breve / metabolism Culture Media / metabolism Female Humans Lactose / analogs & derivatives Lactose / metabolism Milk, Human / microbiology N-Acetylneuraminic Acid / metabolism Neuraminidase / genetics Neuraminidase / metabolism* Oligosaccharides / metabolism* Polysaccharides / metabolism
IF 2.704
Times Cited 8
General Microbes JCM 1275 JCM 1217 JCM 1200 JCM 7019 JCM 1222