| RRC ID |
55057
|
| 著者 |
Mashima I, Djais AA, Haase EM, Scannapieco FA, Otomo M, Saitoh M, Nakazawa F.
|
| タイトル |
Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK.
|
| ジャーナル |
Anaerobe
|
| Abstract |
Recently, Veillonella infantium was isolated from tongue biofilm of a Thai child and established as a novel Veillonella species. In this study, a species-specific primer was designed to identify V. infantium on the basis of the sequence of the 70 kDa heat shock protein (dnaK) gene of Veillonella infantium JCM 31738T (= TSD-88T). The primer pair generated a specific PCR (Polymerase Chain Reaction) product specific for V. infantium, but not for other oral Veillonella species. This specific primer pair could detect dnaK even from 1 pg of genomic DNA extracted from the V. infantium type strain. To validate the primer pair, a number of strains of Veillonella species were isolated from tongue biofilm of 3 Japanese children, DNA was isolated from each strain, and PCR was performed using species-specific primers. All oral Veillonella species except V. infantium were identified by one-step PCR method reported previously. Four kinds of Veillonella species were detected in these subjects. V. rogosae was detected in all subjects and the most predominant species with an average prevalence of 82%. However, V. infantium was detected in 2 of 3 subjects and it was the second most predominant species of oral Veillonella detected in these subjects with an average prevalence of 9.4%. V. infantium appears to coexist with other oral Veillonella species in tongue biofilm. This species-specific primer pair established in this study could be useful to detect V. infantium and support the study of Veillonella for oral health in the future.
|
| 巻・号 |
52
|
| ページ |
79-82
|
| 公開日 |
2018-8-1
|
| DOI |
10.1016/j.anaerobe.2018.06.004
|
| PII |
S1075-9964(18)30107-0
|
| PMID |
29902516
|
| MeSH |
Bacterial Proteins / genetics
Child
Child, Preschool
DNA Primers / genetics*
Female
Gram-Negative Bacterial Infections / microbiology*
HSP70 Heat-Shock Proteins / genetics*
Humans
Male
Phylogeny
Polymerase Chain Reaction
Species Specificity
Veillonella / classification
Veillonella / genetics
Veillonella / isolation & purification*
|
| IF |
2.704
|
| 引用数 |
0
|
| リソース情報 |
| 一般微生物 |
JCM 31738
JCM 17976
JCM 15641
JCM 15642 |
