RRC ID 55058
Author Tsuji R, Yamamoto N, Yamada S, Fujii T, Yamamoto N, Kanauchi O.
Title Induction of anti-viral genes mediated by humoral factors upon stimulation with Lactococcus lactis strain plasma results in repression of dengue virus replication in vitro.
Journal Antiviral Res
Abstract Dengue is a mosquito-borne disease caused by dengue virus (DENV) infection. There is currently no effective vaccine or antiviral treatment available against DENV. In previous studies, we showed that Lactococcus lactis strain Plasma (LC-Plasma) could activate plasmacytoid dendritic cells, which play an important role against virus infection. LC-Plasma administration ameliorated symptoms of viral diseases and its effect appeared to be associated with IFN-α induction. However the precise mechanism of LC-Plasma protection remained unclear. In this study, we investigated the effects of LC-Plasma-induced humoral factors on DENV replication using HepG2 cells as an in vitro infection model. When HepG2 cells were preincubated with supernatants of LC-Plasma-stimulated bone marrow-derived dendritic cells, the replication of DENV was significantly inhibited in a dose dependent manner and its activity was evident regardless of the DENV serotype. In addition, the expression of interferon-stimulated genes, including ISG15, IFITM-1, MxA, RSAD2, and RyDEN, was significantly upregulated by humoral factors. We also compared the effects of representative strains of lactic acid bacteria and found that the ability to prevent DENV replication was unique to LC-Plasma. In addition, it was revealed that both anti-DENV replication activity and ISG induction depended on type I IFN rather than type III IFN signaling. Taken together, since LC-Plasma induces, in a more natural form, potent anti-DENV replication activities irrespective of viral serotypes via induction of type I IFN, LC-Plasma could be safely used as a prophylactic anti-DENV option.
Volume 160
Pages 101-108
Published 2018-12-1
DOI 10.1016/j.antiviral.2018.10.020
PII S0166-3542(18)30474-1
PMID 30393011
MeSH Animals Cell Line Dendritic Cells / immunology* Dengue Virus / growth & development* Dengue Virus / immunology Hep G2 Cells / virology* Humans Immunity, Innate* Immunologic Factors / metabolism* Lactococcus lactis / immunology* Mice, Inbred BALB C Plasma / immunology* Virus Replication / immunology
IF 4.13
Times Cited 0
General Microbes JCM 5805