Reference - Detail
| RRC ID | 55063 |
|---|---|
| Author | Ekimova GA, Fedorov DN, Tani A, Doronina NV, Trotsenko YA. |
| Title | Distribution of 1-aminocyclopropane-1-carboxylate deaminase and D-cysteine desulfhydrase genes among type species of the genus Methylobacterium. |
| Journal | Antonie Van Leeuwenhoek |
| Abstract |
The presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase determines the ability of bacteria to increase the resistance of plants to various types of stress. The genes of ACC deaminase (acdS) and the closely related enzyme D-cysteine desulfhydrase (dcyD) were searched in type strains of various representatives of the genus Methylobacterium. Using PCR screening and in silico searching in the available complete genome sequences of type strains, the genes were found in 28 of 48 species of the genus. Phylogenetic analysis of amino acid sequences of proteins revealed two large groups of sequences of the AcdS protein and one of the DcyD protein. The distribution of these groups correlates well with the phylogenetic tree based on the sequences of the 16S rRNA genes, which apparently indicates a different evolutionary adaptation to association with plants in the representatives of these groups. For the first time for aerobic methylotrophs it was demonstrated that the gene dcyD encodes D-cysteine desulfhydrase by cloning and recombinant protein characterization. |
| Volume | 111(10) |
| Pages | 1723-1734 |
| Published | 2018-10-1 |
| DOI | 10.1007/s10482-018-1061-5 |
| PII | 10.1007/s10482-018-1061-5 |
| PMID | 29520528 |
| MeSH | Carbon-Carbon Lyases / genetics* Carbon-Carbon Lyases / metabolism Cloning, Molecular Cystathionine gamma-Lyase / genetics* Cystathionine gamma-Lyase / metabolism Genes, Bacterial Methylobacterium / classification Methylobacterium / enzymology Methylobacterium / genetics* Phylogeny Plant Growth Regulators / metabolism RNA, Ribosomal, 16S Sequence Analysis, DNA |
| IF | 1.934 |
| Times Cited | 3 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| General Microbes | JCM 10891 JCM 10892 |