RRC ID 55094
著者 Martínez-Tobón DI, Gul M, Elias AL, Sauvageau D.
タイトル Polyhydroxybutyrate (PHB) biodegradation using bacterial strains with demonstrated and predicted PHB depolymerase activity.
ジャーナル Appl Microbiol Biotechnol
Abstract The biodegradation of polyhydroxybutyrate (PHB) has been broadly investigated, but studies typically focus on a single strain or enzyme and little attention has been paid to comparing the interaction of different PHB depolymerase (PhaZ)-producing strains with this biopolymer. In this work, we selected nine bacterial strains-five with demonstrated and four with predicted PhaZ activity-to compare their effectiveness at degrading PHB film provided as sole carbon source. Each of the strains with demonstrated activity were able to use the PHB film (maximum mass losses ranging from 12% after 2 days for Paucimonas lemoignei to 90% after 4 days for Cupriavidus sp.), and to a lower extent Marinobacter algicola DG893 (with a predicted PhaZ) achieved PHB film mass loss of 11% after 2 weeks of exposure. Among the strains with proven PhaZ activity, Ralstonia sp. showed the highest specific activity since less biomass was required to degrade the polymer in comparison to the other strains. In the case of Ralstonia sp., PHB continued to be degraded at pH values as low as pH 3.3-3.7. In addition, analysis of the extracellular fractions of the strains with demonstrated activity showed that Comamonas testosteroni, Cupriavidus sp., and Ralstonia sp. readily degraded both PHB film and PHB particles in agar suspensions. This study highlights that whole cell cultures and enzymatic (extracellular) fractions display different levels of activity, an important factor in the development of PHB-based applications and in understanding the fate of PHB and other PHAs released in the environment. Furthermore, predictions of PhaZ functionality from genome sequencing analyses remain to be validated by experimental results; PHB-degrading ability could not be proven for three of four investigated species predicted by the polyhydroxyalkanoates (PHA) depolymerase engineering database.
巻・号 102(18)
ページ 8049-8067
公開日 2018-9-1
DOI 10.1007/s00253-018-9153-8
PII 10.1007/s00253-018-9153-8
PMID 29951858
MeSH Bacteria / enzymology Bacteria / genetics Bacteria / isolation & purification Bacteria / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Biodegradation, Environmental Carboxylic Ester Hydrolases / genetics Carboxylic Ester Hydrolases / metabolism* Hydroxybutyrates / metabolism*
IF 3.67
引用数 7
リソース情報
一般微生物 JCM 10169 JCM 10168 JCM 10171 JCM 20772 JCM 21637