論文 - 詳細
| RRC ID | 55153 |
|---|---|
| 著者 | Park YU, Jung JH, Seo DH, Jung DH, Kim JH, Seo EJ, Baek NI, Park CS. |
| タイトル | GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity. |
| ジャーナル | Enzyme Microb Technol |
| Abstract |
Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources. We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus. This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa. Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin. Although rDApu showed no activity toward α-cyclodextrin (CD) and β-CD, maltooctaose (G8) was detected from reaction with γ-CD. The highest activity of rDApu was measured at pH 5.0 and 95 °C. The half-life of rDApu was 12.7 h at 95 °C and 27 min at 98 °C. Interestingly, rDApu was able to transfer a maltose unit to 6-O-α-maltosyl-β-CD via transglycosylation. Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 61, 64-di-O-maltosyl-β-CD and 61, 63, 65-tri-O-maltosyl-β-CD. |
| 巻・号 | 114 |
| ページ | 15-21 |
| 公開日 | 2018-7-1 |
| DOI | 10.1016/j.enzmictec.2018.03.005 |
| PII | S0141-0229(18)30117-0 |
| PMID | 29685348 |
| MeSH | Archaeal Proteins / chemistry* Archaeal Proteins / genetics Archaeal Proteins / metabolism Cloning, Molecular Cyclodextrins / chemistry Cyclodextrins / metabolism* Desulfurococcaceae / chemistry Desulfurococcaceae / enzymology* Desulfurococcaceae / genetics Enzyme Stability Glucans / metabolism Glycoside Hydrolases / chemistry* Glycoside Hydrolases / genetics Glycoside Hydrolases / metabolism Glycosylation Maltose / analogs & derivatives Maltose / chemistry Maltose / metabolism Molecular Weight Open Reading Frames Substrate Specificity Trisaccharides / metabolism |
| IF | 2.932 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 9188 |