RRC ID 55189
著者 Hossain TJ, Manabe S, Ito Y, Iida T, Kosono S, Ueda K, Hosomi A, Inoue D, Suzuki T.
タイトル Enrichment and characterization of a bacterial mixture capable of utilizing C-mannosyl tryptophan as a carbon source.
ジャーナル Glycoconj J
Abstract C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excreted via the urine. This paper reports enrichment of a bacterial consortium from soil that degrades CMW. The bacteria grew in minimal medium supplemented with CMW as the carbon source. Interestingly, even after successive clonal picks of individual colonies, several species were still present in each colony as revealed by 16S rRNA gene sequence analysis, indicating that a single species may not be responsible for this activity. A next generation sequencing (NGS) analysis was therefore carried out in order to determine which bacteria were responsible for the catabolism of CMW. It was found that a species of Sphingomonadaceae family, but not others, increased with simultaneous decrease of CMW in the media, suggesting that this species is most likely the one that is actively involved in the degradation of CMW.
巻・号 35(2)
ページ 165-176
公開日 2018-4-1
DOI 10.1007/s10719-017-9807-2
PII 10.1007/s10719-017-9807-2
PMID 29335800
MeSH Biotransformation Microbiota* RNA, Ribosomal, 16S / genetics Soil Microbiology Sphingomonadaceae / genetics Sphingomonadaceae / isolation & purification Sphingomonadaceae / metabolism Tryptophan / analogs & derivatives* Tryptophan / metabolism
IF 2.926
引用数 2
リソース情報
一般微生物 JCM 13185 JCM 9659 JCM 2503