論文 - 詳細
| RRC ID | 55197 |
|---|---|
| 著者 | Teh AH, Chiam NC, Furusawa G, Sudesh K. |
| タイトル | Modelling of polyhydroxyalkanoate synthase from Aquitalea sp. USM4 suggests a novel mechanism for polymer elongation. |
| ジャーナル | Int J Biol Macromol |
| Abstract |
Polyhydroxyalkanoate (PHA) synthase, PhaC, is a key enzyme in the biosynthesis of PHA, a type of bioplastics with huge potential to replace petroleum-based plastics. While two structures have been determined, the exact mechanism remains unclear partly due to the absence of a tunnel for product passage. A model of the class I PhaC from Aquitalea sp. USM4, characterised with Km of 394 μM and kcat of 476 s-1 on 3-(R)-hydroxybutyryl-CoA, revealed a three-branched channel at the dimeric interface. Two of them are opened to the solvent and are expected to serve as the putative routes for substrate entrance and product exit, while the third is elongated in the class II PhaC1 model from Pseudomonas aeruginosa, indicating a role in accommodating the hydroxyalkanoate (HA) moiety of a HA-CoA substrate. Docking of the two tetrahedral intermediates, formed during the transfer of the growing PHA chain from the catalytic Cys to a new molecule of substrate and back to Cys, suggests a common elongation mechanism requiring the HA moiety of the ligand to rotate ~180°. Substrate specificity is determined in part by a bulky Phe/Tyr/Trp residue in the third branch in class I, which is conserved as Ala in class II to create room for longer substrates. |
| 巻・号 | 119 |
| ページ | 438-445 |
| 公開日 | 2018-11-1 |
| DOI | 10.1016/j.ijbiomac.2018.07.147 |
| PII | S0141-8130(18)32077-4 |
| PMID | 30048726 |
| MeSH | Acyltransferases / chemistry* Acyltransferases / metabolism* Betaproteobacteria / enzymology* Models, Molecular* Polyhydroxyalkanoates / chemistry* Polyhydroxyalkanoates / metabolism* Polymerization Protein Conformation Substrate Specificity |
| IF | 4.784 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 19919 |