| RRC ID |
55428
|
| 著者 |
Kitagawa W, Mitsuhashi S, Hata M, Tamura T.
|
| タイトル |
Identification of a novel bacteriocin-like protein and structural gene from Rhodococcus erythropolis JCM 2895, using suppression-subtractive hybridization.
|
| ジャーナル |
J Antibiot (Tokyo)
|
| Abstract |
A novel bacteriocin-like protein and its structural gene (rap) were identified from Rhodococcus erythropolis JCM 2895. The rapA and B genes are located on a 5.4-kb circular plasmid, and were obtained using a modified suppression-subtractive hybridization method. The rapA and B genes were heterologously expressed in Rhodococcus sp. or Escherichia coli, and then characterized. The results indicated that RapA is a small, water-soluble, heat-stable antimicrobial protein, and that RapB is an immunity protein against RapA, estimated to be located on the cell membrane. RapA showed antimicrobial activity particularly against R. erythropolis, and the activity persisted even after SDS-PAGE analysis. For the heterologous expressed RapA protein, N-terminal amino acid sequence was also confirmed. This is the first report of a bacteriocin-like substance obtained from the genus Rhodococcus.
|
| 巻・号 |
71(10)
|
| ページ |
872-879
|
| 公開日 |
2018-10-1
|
| DOI |
10.1038/s41429-018-0078-3
|
| PII |
10.1038/s41429-018-0078-3
|
| PMID |
29980745
|
| MeSH |
Bacteriocins / chemistry
Bacteriocins / genetics
Bacteriocins / metabolism*
Gene Expression Regulation, Bacterial / physiology*
Rhodococcus / metabolism*
|
| IF |
2.446
|
| 引用数 |
3
|
| リソース情報 |
| 一般微生物 |
JCM 2895
JCM 2893
JCM 2894
JCM 20154
JCM 20186
JCM 3201 |
