| RRC ID |
55440
|
| 著者 |
Saika A, Utashima Y, Koike H, Yamamoto S, Kishimoto T, Fukuoka T, Morita T.
|
| タイトル |
Identification of the gene PtMAT1 encoding acetyltransferase from the diastereomer type of mannosylerythritol lipid-B producer Pseudozyma tsukubaensis.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
Mannosylerythritol lipids (MELs) are biosurfactants produced from feedstocks by basidiomycetous yeasts. MELs exhibit different properties depending on their structures, such as the degree of acetylation or acylation and the chirality of the mannosylerythritol moiety. Pseudozyma tsukubaensis produces a diastereomer type of MEL-B (mono-acetylated MEL); therefore, deletion of an acetyltransferase could yield a diastereomer type of MEL-D (deacetylated MEL), which has only been produced in in vitro reactions of lipase using MEL-B as a substrate. Here, we deleted the gene PtMAT1 in P. tsukubaensis 1E5 encoding an acetyltransferase related to MEL biosynthesis via targeted gene deletion and generated a producer of the diastereomer type of MEL-D. The uracil auxotrophic mutant of P. tsukubaensis 1E5 (PtURA5-mutant) was used as a host strain for gene deletion. The gene PtMAT1 was replaced with a PtURA5 cassette by homologous recombination using uracil auxotrophy as a selectable marker. According to thin-layer chromatography and nuclear magnetic resonation spectroscopy, we identified the strain ΔPtMAT1 as a producer of the diastereomer type of MEL-D instead of MEL-B.
|
| 巻・号 |
126(6)
|
| ページ |
676-681
|
| 公開日 |
2018-12-1
|
| DOI |
10.1016/j.jbiosc.2018.05.025
|
| PII |
S1389-1723(18)30026-4
|
| PMID |
30037643
|
| MeSH |
Acetyltransferases / genetics*
Acetyltransferases / isolation & purification
Acylation
Chromatography, Thin Layer
Cloning, Molecular
Genes, Fungal
Glycolipids / biosynthesis*
Glycolipids / chemistry
Glycolipids / metabolism
Magnetic Resonance Spectroscopy
Stereoisomerism
Surface-Active Agents / chemistry
Surface-Active Agents / metabolism
Ustilaginales / genetics*
Ustilaginales / metabolism*
|
| IF |
2.032
|
| 引用数 |
0
|
| リソース情報 |
| 一般微生物 |
JCM 16987 |
