| RRC ID |
55469
|
| 著者 |
Takajo I, Yamada A, Umeki K, Saeki Y, Hashikura Y, Yamamoto I, Umekita K, Urayama-Kawano M, Yamasaki S, Taniguchi T, Misawa N, Okayama A.
|
| タイトル |
Development of a simple and practical method of discrimination between Vibrio furnissii and V. fluvialis based on single-nucleotide polymorphisms of 16S rRNA genes observed in V. furnissii but not in V. fluvialis.
|
| ジャーナル |
J Microbiol Methods
|
| Abstract |
Vibrio furnissii and V. fluvialis are closely related, the discrimination of which by conventional biochemical assay remains a challenge. Investigation of the sequence of the 16S rRNA genes in a clinical isolate of V. furnissii by visual inspection of a sequencing electropherogram revealed two sites of single-nucleotide polymorphisms (SNPs; positions 460 A/G and 1261 A/G) in these genes. A test of 12 strains each of V. fluvialis and V. furnissii revealed these SNPs to be common in V. furnissii but not in V. fluvialis. Divergence of SNP frequency was observed among the strains of V. furnissii tested. Because the SNPs described in V. furnissii produce a difference in the target sequence of restriction enzymes, a combination of polymerase chain reaction (PCR) of the 16S rRNA genes using conventional primers and restriction fragment length polymorphism analysis using Eco RV and Eae I was shown to discriminate between V. fluvialis and V. furnissii. This method is simple and alleviates the need for expensive equipment or primer sets specific to these bacteria. Therefore, we believe that this method can be useful, alongside specific PCR and mass spectrometry, when there is a need to discriminate between V. fluvialis and V. furnissii.
|
| 巻・号 |
144
|
| ページ |
22-28
|
| 公開日 |
2018-1-1
|
| DOI |
10.1016/j.mimet.2017.10.014
|
| PII |
S0167-7012(17)30279-8
|
| PMID |
29111399
|
| MeSH |
Bacterial Typing Techniques / methods*
Base Sequence
Genes, Bacterial / genetics
Mass Spectrometry / methods
Molecular Diagnostic Techniques / methods
Polymerase Chain Reaction / methods
Polymorphism, Single Nucleotide / genetics*
RNA, Ribosomal, 16S / genetics*
Sequence Analysis, DNA
Species Specificity
Vibrio / classification*
Vibrio / genetics*
Vibrio / isolation & purification*
Water Microbiology
|
| IF |
1.701
|
| 引用数 |
1
|
| リソース情報 |
| 一般微生物 |
JCM 1281
JCM 1282 |
