RRC ID 55475
Author Yamada R, Tien LHT, Arai S, Tohya M, Ishida-Kuroki K, Nomoto R, Kim H, Suzuki E, Osawa R, Watanabe T, Sekizaki T.
Title Development of PCR for identifying Streptococcus parasuis, a close relative of Streptococcus suis.
Journal J. Vet. Med. Sci.
Abstract Streptococcus parasuis has recently been removed taxonomically from Streptococcus suis, a zoonotic pathogen. S. parasuis has been detected in healthy pigs and in diseased pigs, which suggests that S. parasuis is involved in the normal microbiota of pigs and has potential pathogenicity. However, the pathogenicity of S. parasuis in pigs is unclear because of the lack of appropriate detection methods that discriminate S. parasuis from S. suis. In this study, we developed a PCR method that is specific for S. parasuis. The detection limit of the PCR was 350 CFU per reaction. Bacteria isolated from the saliva of eight pigs were collected and examined by PCR. Sixty-four isolates positive for PCR were obtained from the samples of all pigs. Thirteen of the 64 isolates were genetically confirmed as S. parasuis, and biologically and biochemically had nearly the same features of known S. parasuis strains, which suggested that strains positive for PCR were S. parasuis. Among the 64 isolates, 28 isolates were serotypes 20, 22, or 26 in the S. suis serotyping scheme. The remaining 36 isolates were untypeable, which suggested the presence of novel serotypes or a capsule-negative form. Therefore, the PCR method described in this study is a useful tool for identifying S. parasuis, and can be used in etiological studies on this bacterium.
Volume 80(7)
Pages 1101-1107
Published 2018-7-12
DOI 10.1292/jvms.18-0083
PMID 29877313
PMC PMC6068303
MeSH Animals Polymerase Chain Reaction / veterinary* Saliva / microbiology* Serotyping Streptococcal Infections / microbiology Streptococcal Infections / veterinary* Streptococcus suis / classification* Streptococcus suis / isolation & purification Swine Swine Diseases / microbiology*
IF 0.803
Resource
General Microbes JCM 12180 JCM 12181 JCM 12997