RRC ID 5549
Author Araki K, Takeda N, Yoshiki A, Obata Y, Nakagata N, Shiroishi T, Moriwaki K, Yamamura K.
Title Establishment of germline-competent embryonic stem cell lines from the MSM/Ms strain.
Journal Mamm. Genome
Abstract MSM/Ms is an inbred mouse strain established from the Japanese wild mouse, Mus musculus molossinus, which has been phylogenetically distinct from common laboratory mouse strains for about 1 million years. The nucleotide substitution rate between MSM/Ms and C57BL/6 is estimated to be 0.96%. MSM/Ms mice display unique characteristics not observed in the commonly used laboratory strains, including an extremely low incidence of tumor development, high locomotor activity, and resistance to high-fat-diet-induced diabetes. Thus, functional genomic analyses using MSM/Ms should provide a powerful tool for the identification of novel phenotypes and gene functions. We report here the derivation of germline-competent embryonic stem (ES) cell lines from MSM/Ms blastocysts, allowing genetic manipulation of the M. m. molossinus genome. Fifteen blastocysts were cultured in ES cell medium and three ES lines, Mol/MSM-1, -2, and -3, were established. They were tested for germline competency by aggregation with ICR morulae and germline chimeras were obtained from all three lines. We also injected Mol/MSM-1 ES cells into blastocysts of ICR or C57BL/6 x BDF1 mice and found that blastocyst injection resulted in a higher production rate of chimeric mice than did aggregation. Furthermore, Mol/MSM-1 subclones electroporated with a gene trap vector were also highly efficient at producing germline chimeras using C57BL/6 x BDF1 blastocyst injection. This Mol/MSM-1 ES line should provide an excellent new tool allowing the genetic manipulation of the MSM/Ms genome.
Volume 20(1)
Pages 14-20
Published 2009-1
DOI 10.1007/s00335-008-9160-7
PMID 19082856
MeSH Animals Animals, Wild / embryology Cell Differentiation / physiology Cell Line* Chimera / embryology Crosses, Genetic Electroporation Embryo Culture Techniques Embryonic Stem Cells / cytology* Embryonic Stem Cells / physiology* Female Germ Cells / physiology* Male Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Inbred Strains
IF 2.687
Times Cited 9