論文 - 詳細
| RRC ID | 55493 |
|---|---|
| 著者 | Wang J, Liu Y, Liu Y, Du K, Xu S, Wang Y, Krupovic M, Chen X. |
| タイトル | A novel family of tyrosine integrases encoded by the temperate pleolipovirus SNJ2. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Genomes of halophilic archaea typically contain multiple loci of integrated mobile genetic elements (MGEs). Despite the abundance of these elements, however, mechanisms underlying their site-specific integration and excision have not been investigated. Here, we identified and characterized a novel recombination system encoded by the temperate pleolipovirus SNJ2, which infects haloarchaeon Natrinema sp. J7-1. SNJ2 genome is inserted into the tRNAMet gene and flanked by 14 bp direct repeats corresponding to attachment core sites. We showed that SNJ2 encodes an integrase (IntSNJ2) that excises the proviral genome from its host cell chromosome, but requires two small accessory proteins, Orf2 and Orf3, for integration. These proteins were co-transcribed with IntSNJ2 to form an operon. Homology searches showed that IntSNJ2-type integrases are widespread in haloarchaeal genomes and are associated with various integrated MGEs. Importantly, we confirmed that SNJ2-like recombination systems are encoded by haloarchaea from three different genera and are critical for integration and excision. Finally, phylogenetic analysis suggested that IntSNJ2-type recombinases belong to a novel family of archaeal integrases distinct from previously characterized recombinases, including those from the archaeal SSV- and pNOB8-type families. |
| 巻・号 | 46(5) |
| ページ | 2521-2536 |
| 公開日 | 2018-3-16 |
| DOI | 10.1093/nar/gky005 |
| PII | 4816213 |
| PMID | 29361162 |
| PMC | PMC5861418 |
| MeSH | Archaea / enzymology Archaea / genetics Chromosomes, Archaeal Integrases / biosynthesis Integrases / classification Integrases / genetics Integrases / metabolism* Interspersed Repetitive Sequences Proviruses / physiology Recombination, Genetic Transcription, Genetic Tyrosine Viral Proteins / biosynthesis Viral Proteins / genetics Viral Proteins / physiology Virus Integration Viruses / enzymology* Viruses / genetics |
| IF | 11.147 |
| 引用数 | 13 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10635 JCM 10478 |