RRC ID |
55496
|
著者 |
Arai S, Iwabuchi N, Takahashi S, Xiao JZ, Abe F, Hachimura S.
|
タイトル |
Orally administered heat-killed Lactobacillus paracasei MCC1849 enhances antigen-specific IgA secretion and induces follicular helper T cells in mice.
|
ジャーナル |
PLoS One
|
Abstract |
Antigen-specific immunoglobulin (Ig) A plays a major role in host defense against infections in gut mucosal tissue. Follicular helper T (Tfh) cells are located in germinal centers and promote IgA production via interactions with germinal center B cells. Several studies have demonstrated that some lactic acid bacteria (LAB) strains activate the host's acquired immune system, inducing IgA secretion in the intestine. However, the precise molecular mechanisms underlying the effects of LAB on IgA production and Tfh cells are not fully resolved. Lactobacillus paracasei MCC1849 is a probiotic strain isolated from the intestine of a healthy adult. In this study, we investigated the effects of orally administered heat-killed MCC1849 on IgA production in the intestine and on Tfh cell induction in vivo. We found that orally administered MCC1849 induced antigen-specific IgA production in the small intestine, serum and lungs. We also observed that MCC1849 increased the proportion of IgA+ B cells and Tfh cells in Peyer's patches (PPs). In addition, MCC1849 increased the gene expression of IL-12p40, IL-10, IL-21, STAT4 and Bcl-6 associated with Tfh cell differentiation. These results suggest that orally administered MCC1849 enhances antigen-specific IgA production and likely affects Tfh cell differentiation in PPs.
|
巻・号 |
13(6)
|
ページ |
e0199018
|
公開日 |
2018-6-13
|
DOI |
10.1371/journal.pone.0199018
|
PII |
PONE-D-17-34634
|
PMID |
29897995
|
PMC |
PMC5999281
|
MeSH |
Administration, Oral
Animals
B-Lymphocytes / cytology
B-Lymphocytes / metabolism
Cell Differentiation
Cytokines / analysis
Cytokines / metabolism
Hot Temperature
Immunoglobulin A, Secretory / blood
Immunoglobulin A, Secretory / metabolism*
Influenza A Virus, H1N1 Subtype / pathogenicity
Intestine, Small / metabolism
Lacticaseibacillus paracasei / immunology*
Lung / metabolism
Male
Mice
Mice, Inbred BALB C
Orthomyxoviridae Infections / prevention & control
Ovalbumin / immunology
Peyer's Patches / metabolism
Probiotics / administration & dosage
Proto-Oncogene Proteins c-bcl-6 / metabolism
T-Lymphocytes, Helper-Inducer / cytology
T-Lymphocytes, Helper-Inducer / immunology*
T-Lymphocytes, Helper-Inducer / metabolism
|
IF |
2.776
|
引用数 |
9
|
リソース情報 |
一般微生物 |
JCM 1248
JCM 1134
JCM 1112
JCM 1185
JCM 8130
JCM 1120 |