RRC ID 55496
著者 Arai S, Iwabuchi N, Takahashi S, Xiao JZ, Abe F, Hachimura S.
タイトル Orally administered heat-killed Lactobacillus paracasei MCC1849 enhances antigen-specific IgA secretion and induces follicular helper T cells in mice.
ジャーナル PLoS One
Abstract Antigen-specific immunoglobulin (Ig) A plays a major role in host defense against infections in gut mucosal tissue. Follicular helper T (Tfh) cells are located in germinal centers and promote IgA production via interactions with germinal center B cells. Several studies have demonstrated that some lactic acid bacteria (LAB) strains activate the host's acquired immune system, inducing IgA secretion in the intestine. However, the precise molecular mechanisms underlying the effects of LAB on IgA production and Tfh cells are not fully resolved. Lactobacillus paracasei MCC1849 is a probiotic strain isolated from the intestine of a healthy adult. In this study, we investigated the effects of orally administered heat-killed MCC1849 on IgA production in the intestine and on Tfh cell induction in vivo. We found that orally administered MCC1849 induced antigen-specific IgA production in the small intestine, serum and lungs. We also observed that MCC1849 increased the proportion of IgA+ B cells and Tfh cells in Peyer's patches (PPs). In addition, MCC1849 increased the gene expression of IL-12p40, IL-10, IL-21, STAT4 and Bcl-6 associated with Tfh cell differentiation. These results suggest that orally administered MCC1849 enhances antigen-specific IgA production and likely affects Tfh cell differentiation in PPs.
巻・号 13(6)
ページ e0199018
公開日 2018-6-13
DOI 10.1371/journal.pone.0199018
PII PONE-D-17-34634
PMID 29897995
PMC PMC5999281
MeSH Administration, Oral Animals B-Lymphocytes / cytology B-Lymphocytes / metabolism Cell Differentiation Cytokines / analysis Cytokines / metabolism Hot Temperature Immunoglobulin A, Secretory / blood Immunoglobulin A, Secretory / metabolism* Influenza A Virus, H1N1 Subtype / pathogenicity Intestine, Small / metabolism Lacticaseibacillus paracasei / immunology* Lung / metabolism Male Mice Mice, Inbred BALB C Orthomyxoviridae Infections / prevention & control Ovalbumin / immunology Peyer's Patches / metabolism Probiotics / administration & dosage Proto-Oncogene Proteins c-bcl-6 / metabolism T-Lymphocytes, Helper-Inducer / cytology T-Lymphocytes, Helper-Inducer / immunology* T-Lymphocytes, Helper-Inducer / metabolism
IF 2.776
引用数 9
リソース情報
一般微生物 JCM 1248 JCM 1134 JCM 1112 JCM 1185 JCM 8130 JCM 1120